ZORBAX column family is one of the most popular HPLC column families for reversed-phase HPLC. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution. From ZORBAX Eclipse Plus C18 to sub-2 micron UHPLC columns, a wide selection of stationary phases and particle sizes allows you to fine-tune your selectivity to match your application. Features • Traditional, fully porous particles available in 1.8, 3.5, and 5 µm sizes. With selected products also available in 7 μm particles for preparative HPLC (see Prep LC columns section) • ZORBAX Eclipse Plus columns are the recommended starting point for method development and benefit from the ultimate performance from fully porous silica-based columns • ZORBAX StableBond columns offer the highest stability for low pH applications down to pH 1 while ZORBAX SB-Aq deliver robust, reproducible separations with highly aqueous mobile phases, including 100% water • ZORBAX Extend-C18 columns offer high efficiency for high pH applications up to pH 11.5 • ZORBAX Bonus RP columns offer unique selectivity due to an embedded amide linkage in the C14-alkyl chain making them ideal for 100% aqueous mobile phases and for compounds that are not retained on standard reverse phase columns • ZORBAX Rx columns are made from highly pure (> 99.995 %) porous silica with a higher carbon load (12%) • ZORBAX Eclipse PAH columns deliver high resolution separation of the 16 PAHs in EPA 610 and have been specifically tested with PAHs for maximum reproducibility
Method Validation Kits for Biomolecules
ZORBAX Aqueous Method Development Kit, includes ZORBAX Bonus-RP, SB-Aq, and SB-C18 columns
AdvanceBio HIC LC column. method validation kit, includes three columns from different batches
InfinityLab Poroshell 120-LC column with column ID
InfinityLab Poroshell 120-LC column with column ID
InfinityLab Poroshell 120-LC column with column ID
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations. Features • Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins • Exceptional stability and long lifetime at low pH with TFA containing mobile phases • Excellent reproducibility • Available in capillary and nano column dimensions for proteomics and other LC/MS applications
InfinityLab Poroshell 120 LC column with column ID.
InfinityLab Poroshell 120 LC column with column ID.
InfinityLab Poroshell 120 LC column with column ID.
InfinityLab Poroshell 120 LC column with column ID.
Agilent Omnispher C18, Chromsep includes holder with guard
Microsorb 100-3 C18 30 x 4.6mm Conv., 0.5 ml tube, 0.3 ml solvent
ZORBAX RRHD Eclipse Plus C18, with column ID tag.
PEEK-lined reversed-phase columns specifically designed for analysis of post-translational modifications (PTMs) at the peptide level.
AdvanceBio Peptide Plus columns are reversed-phase, superficially porous particle HPLC columns optimized for the separation of target peptides, impurities, and post translational modifications. These columns feature a hybrid endcapped C18 stationary phase on a 100 Å pore size, 2.7 μm particle modified to have a charged surface. AdvanceBio Peptide Plus columns enable you to identify multiple critical quality attributes confidently by LC/MS. They provide the speed, sensitivity, and efficiency you need to quickly confirm and identify target proteins and peptides.
InfinityLab Poroshell 120, with column ID tag, PEEK-lined.
InfinityLab Poroshell 120 superficially porous columns for reversed-phase LC separations offer exceptional efficiency and reliability. Eleven chemistries - from a C18 column to unique chemistries - are available in up to three particle diameters: 1.9 μm, 2.7 μm, and 4 μm. This range of chemistries provides a broad choice of selectivity for scalable LC method development, from traditional HPLC to UHPLC and to ultralow dispersion UHPLC.
The AdvanceBio SEC 1.9 µm columns are designed to improve resolution and provided faster separation of protein aggregates and fragments by UHPLC. The optimized sub-two-micron particles are monodispersed and modified by unique hydrophilic bonding chemistry providing column reproducibility, stability as well as preventing secondary interactions and protein adsorption. The AdvanceBio SEC 1.9 μm column is intended to meet the needs for qualitative and quantitative critical quality attributes (CQA) analysis and method transferability across departments.
InfinityLab PFC Delay Column, 4.6 x 30 mm. 1200 bar pressure limit; designed and tested to delay elution of PFC compounds released from pump, solvent lines and solvents - to be used with InfinityLab PFC-free LC Conversion Kit (5004-0006)
Kethod validation kit, includes three columns from different batches
InfinityLab Poroshell 120-LC column with column ID InfinityLab Poroshell 120 superficially porous columns for reversed-phase liquid chromatography (LC) separations. Features: • Superficially porous, core-shell particles that provide both improved throughput and higher resolution • A scalable family of particles - including 1.9 μm, 2.7 μm, and 4 μm - for the best performance from your methods and instruments • Superior peak shapes for faster, more accurate results due to high-purity silica and advanced bonding chemistries • Excellent lot-to-lot reproducibility giving you confidence in your separation results • Part of the InfinityLab family – designed for ease-of-use and maximum compatibility with Agilent instruments and supplies • Long column life with uniquely robust 1.9 μm low-dispersion UHPLC columns • Reduced need for sample preparation due to 2 μm frits on 2.7 μm and 4 μm columns • UHPLC guard columns to further extend the life of your analytical column • Scalable to Preparative LC dimensions with the SB-C18 and HPH-C18 stationary phases • Enhanced usability, traceability, and security with a preprogrammed ID tag (Part Numbers with the T suffix, denote the included ID tag option)
InfinityLab Poroshell 120, Reversed Phase HPLC Columns
InfinityLab Reversed Phase Prep Any purification campaign centers around three objectives – yield, purity, and throughput. Agilent InfinityLab preparative LC columns are optimized to help you to achieve your goals. Available in a variety of media, such as Pursuit XRs with high loading capacity, ZORBAX with diverse selectivity, or Poroshell 120 with high speed and efficiency these columns offer a suitable fit for your purification needs. Agilent InfinityLab preparative LC columns are available in 21.2 and 30 mm internal diameters and easily scale from analytical dimensions.
InfinityLab Reversed Phase Prep HPLC Column. Any purification campaign centers around three objectives – yield, purity, and throughput. Agilent InfinityLab preparative LC columns are optimized to help you to achieve your goals. Available in a variety of media, such as Pursuit XRs with high loading capacity, ZORBAX with diverse selectivity, or Poroshell 120 with high speed and efficiency these columns offer a suitable fit for your purification needs. Agilent InfinityLab preparative LC columns are available in 21.2 and 30 mm internal diameters and easily scale from analytical dimensions.
InfinityLab Pursuit XRs preparative LC columns for bulk purifications
InfinityLab Reversed Phase Prep InfinityLab Poroshell 120 LC column
InfinityLab ZORBAX preparative LC columns for high throughput and bulk purifications
InfinityLab ZORBAX preparative LC columns for high throughput and bulk purifications
InfinityLab Pursuit XRs Diphenyl. Preparative HPLC column utilizes strong dipole-dipole hydrogen bonding and pi-pi mechanisms for different selectivity with aromatic compounds.
InfinityLab Pursuit XRs preparative LC columns for bulk purifications
InfinityLab ZORBAX präparative LC-Säulen für Hochdurchsatz- und Massenaufreinigungen
InfinityLab Poroshell 120 superficially porous columns for reversed-phase liquid chromatography (LC) separations offer exceptional efficiency and reliability. Twenty chemistries - from various C18 columns to other unique phases - are available in up to three particle diameters: 1.9 μm, 2.7 μm, and 4 μm. This range of chemistries provides a broad choice of selectivity for scalable LC method development, from traditional high-performance liquid chromatography (HPLC) to ultra-high-performance liquid chromatography (UHPLC) and to ultralow dispersion UHPLC.
EC HPLC column. Reversed phase with polar selectivity for organic acid analysis. Suitability for organic acid analysis is assured through qualified batch analysis. This HPLC phase is made of high purity NUCLEODUR HPLC silica gel and suitable for usage with 100% aqueous mobile phase.
EC HPLC column NUCLEOSHELL Bluebird RP 18 The stationary phase is based on core-shell technology silica gel particles and is modified by an octadecyl modification with polar endcapping. Polar endcapping makes this C18 phase suitable for aqueous eluents. Recommended applications: Nitrosamines, Organic acids, Pesticides, Pharmaceuticals, Reversed phase, RP applications HPLC, Sweeteners, Very polar analytes, Water-soluble vitamins
EC HPLC column EC 150/4.6 NUCLEOSHELL Biphenyl, 2.7 µm length: 150 mm, ID: 4.6 mm pack of 1
EC HPLC column. Hydrophobic phase with alternative selectivity compared to classical C18 modifications. The separation principle based on two retention mechanisms - π-π interactions and hydrophobic interactions. This provides better retention of aromatic and unsaturated substances. This stationary HPLC phase is based on high purity NUCLEODUR silica gel and shows excellent performance under highly aqueous conditions. Recommended applications: Antibiotics, Especially aromatic and unsaturated compounds, Pharmaceuticals, Polar compounds, Reversed phase, RP applications HPLC, Steroids
Analytical EC column. Hydrophobic reversed phase with distinct polar selectivity for special PFAS analysis. Suitability for PFAS analysis is assured through qualified batch analysis. This HPLC phase is made of high purity NUCLEODUR HPLC silica gel and is extensively endcapped. Due to a low bleeding characteristics these columns are suitable for high sensitivity LC/MS analysis.
NUCLEODUR PFAS Delay columns trap and therefor delay system-related PFAS contaminants, ensuring trace level analysis of PFAS in your samples. This HPLC phase is made of high purity NUCLEODUR HPLC silica gel and is extensively endcapped. Due to a low bleeding characteristics these columns are suitable for high sensitivity LC/MS analysis.
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