Eclipse XDB Columns • provide excellent separations over a wide pH range (pH 2 to pH 9), • provide reliable performance under high pressure and flow - a result of Agilent's proprietary ZORBAX silica gel particles, • enable flexible method development with many choices in four bonded phases and a wide range of particle sizes and dimensions.
Eclipse XDB Columns • provide excellent separations over a wide pH range (pH 2 to pH 9), • provide reliable performance under high pressure and flow - a result of Agilent's proprietary ZORBAX silica gel particles, • enable flexible method development with many choices in four bonded phases and a wide range of particle sizes and dimensions.
The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed-phase HPLC. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution. Features • Traditional, fully porous particles available in 1.8, 3.5, and 5 µm sizes. With selected products also available in 7 μm particles for preparative HPLC • ZORBAX Extend-C18 columns offer high efficiency for high pH applications up to pH 11.5
ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series. The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions. The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation. Features • High efficiency and reproducibility with short analysis time • Semi-prep and prep column dimensions • Compatible with organic modifiers and denaturants • Wide usable pH range (3-8)
ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series. The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions. The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation. Features • High efficiency and reproducibility with short analysis time • Semi-prep and prep column dimensions • Compatible with organic modifiers and denaturants • Wide usable pH range (3-8)
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