XTerra Preparative RP18 columns are hybrid-based, reversed-phase C18 columns that incorporate Waters patented embedded polar group technology to improve peak shape performance for highly basic compounds. These first generation columns provide superior pH stability compared to a silica-based column.
Symmetry C8 columns feature monofunctionally bonded C8 ligands on a high purity base-deactived silica. Symmetry columns have the tightest column specifications in the industry giving resulting highly reproducible chromatographic results.
The L1 UPLC method development kit combines 1.7 µm BEH C18, Shield RP18, and 1.8 µm HSS C18 and T3 column chemistries to accomodate your method development approach, enabling methods to be developed efficiently and effectively. By utilizing UPLC Technology for method development, a substantial improvement in throughput can be realized. This, in turn, reduces cost per sample and time of analysis considerably while maintaining or improving separation integrity, enabling products to be brought to market faster and improving the overall profitability of the assay.
Rigid, hydrophilic, polymethacrylate 8 and 15 µm particles with 1000Å pores.
The separation of proteins and peptides based upon hydrophobic characteristics is a powerful chromatographic technique. However, some proteins denature at elevated organic solvent concentrations making reversed-phase chromatography (RPC) difficult. Waters BioSuite™ Phenyl HIC columns provide a viable separation alternative to RPC. HIC is characterized by the adsorption of compounds to a weakly hydrophobic surface at high salt concentrations, followed by elution with a decreasing salt gradient. HIC combines the non-denaturing characteristics of salt precipitation with the precision of HPLC to yield excellent separation of biologically active material. Waters BioSuite™ Phenyl, 1000, 10 µm HIC column media consists of a phenyl group bonded to a methacrylic ester-based polymeric resin. The large 1000 angstrom pore size accommodates proteins up to 5,000,000 Daltons.
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