• Rugged, high and low pH separations of polypeptides and peptides from pH 2-11.5 • Different selectivity possible at high and low pH • High efficiency and good recovery of hydrophobic peptides at high pH • Ideal for LC/MS with ammonium-hydroxide-modified mobile phase Agilent ZORBAX 300Å Extend-C18 is a wide-pore HPLC column for high efficiency separations of peptides from pH 2-11.5. The unique, bidentate bonded phase provides excellent lifetime and reproducibility at high and low pH. At high pH, retention and selectivity of peptides and polypeptides can change dramatically as a result of changes in charge on molecules. Excellent recoveries of hydrophobic polypeptides have been achieved at room temperature and high pH. LC/MS sensitivity of peptides and polypeptides can also be improved at high pH using a simple ammonium-hydroxide-containing mobile phase.
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations.
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations.
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations. Features • Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins • Exceptional stability and long lifetime at low pH with TFA containing mobile phases • Excellent reproducibility • Available in capillary and nano column dimensions for proteomics and other LC/MS applications
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations. Features • Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins • Exceptional stability and long lifetime at low pH with TFA containing mobile phases • Excellent reproducibility • Available in capillary and nano column dimensions for proteomics and other LC/MS applications
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations. Features • Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins • Exceptional stability and long lifetime at low pH with TFA containing mobile phases • Excellent reproducibility • Available in capillary and nano column dimensions for proteomics and other LC/MS applications
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations. Features • Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins • Exceptional stability and long lifetime at low pH with TFA containing mobile phases • Excellent reproducibility • Available in capillary and nano column dimensions for proteomics and other LC/MS applications
ZORBAX Ion Exchange columns are available as both Strong Anion-Exchange (SAX) and Strong Cation-Exchange (300SCX) columns. Each column is packed with bonded, 5 µm, spherical silica particles for optimum efficiency in separating and purifying biological macromolecules, including proteins, peptides and polynucleotides. ZORBAX Ion Exchange columns exhibit a high degree of mechanical stability. Therefore, they can be used at high operating flow rates and pressures. The columns are compatible with rapid changes in ionic strength, thus short gradient times and rapid re-equilibration are possible. Features • ZORBAX SAX and 300SCX columns are based on rugged ZORBAX silica • Stable from pH 2–7 • Provide high efficiency, rapid separations • Compatible with organic mobile phase modifiers
AdvanceBio Glycan Mapping columns are designed and manufactured to deliver fast, high resolution, reproducible glycan identification using HILIC chromatography. Two UHPLC configurations are available: 2.7 µm superficially porous, for high resolution and lower backpressure, and 1.8 µm for highest resolution. AdvanceBio Glycan Mapping columns leverage technology that optimizes results for MS and fluorescence detection. They can deliver glycan maps in less than 10 minutes - 40% less time than competing products - and can be ordered with a selection of standards for performance testing and retention mapping of labeled and unlabeled glycans.
AdvanceBio Oligonucleotide columns offer high-quality, high-resolution ion-pair reversed-phase (IP-RP) column chemistry platform that support analytical characterization through purification with seamless method scale-up. To successfully separate trityl-off, deprotected oligonucleotides, HPLC columns need to have high resolving power and be robust enough to withstand high pH and temperature, which provide better separation and denaturing conditions for optimal resolution and oligonucleotide separation. AdvanceBio oligonucleotide columns feature high efficiency, 2.7 and 4 μm superficially porous Poroshell particles. Using technology exclusive to Agilent, we chemically modify the particles to make them exceedingly resistant to high pH mobile phases. We also bond them with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides. In addition, we test every batch of AdvanceBio oligonucleotide media with a resolution standard to ensure consistent performance.AdvanceBio products are designed to deliver consistent, exceptional performance for the complete characterization and bioanalysis of proteins, antibodies, conjugates, new biological entities and biopharmaceuticals.
Agilent AdvanceBio Peptide Mapping columns are designed to provide high-resolution peptide maps for protein identification and determination of post translation modifications. These columns provide the ability for you to quickly resolve and identify amino acid substitutions/modifications in a protein primary sequence. These advanced biocolumns are superficially porous and have a 120 Å pore size, which is optimum for the analysis of the peptides produced by enzymatic digest of proteins. The 2.7 µm particle size enables UHPLC performance on HPLC systems, which delivers high resolution separations 2–3 times faster than with fully porous HPLC columns. Each batch of the AdvanceBio Peptide Mapping media is tested with a peptide mix to ensure suitability and reproducibility, and to provide greater confidence in the analytical data generated. Use Agilent’s 10-Peptide Quality Control Standard, the same standard Agilent uses to QC its columns, to evaluate your column’s performance over its lifetime. It can be used for HPLC or LC/MS. Approximately 20 injections per vial. Order part number 5190-0583. AdvanceBio Peptide Mapping columns are the first members of Agilent’s growing state-of-the-art family of biocolumns. They are designed to deliver consistent, exceptional performance for the separation and characterization of peptides and proteins.
AdvanceBio RP-mAb columns are designed to optimize the performance of intact and reduced mAb analysis when analyzing monoclonal antibodies for biopharma discovery, development, and QA/QC applications. Analysis of intact and reduced monoclonal antibodies are critical measurements for characterizing therapeutic proteins and understanding their efficacy and stability. Poor chromatographic separations can result in rework and even compromise the accuracy of the characterization. Based on Poroshell technology with unique engineering for pore size and bonded phases, AdvanceBio RP-mAb columns deliver higher resolution and faster run times to provide accurate, reproducible results. Available in a range of chemistries – SB-C8, C4, and diphenyl. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers . Features: • Improved accuracy: Superficially porous particles (3.5 µm) with wide pores (450Å) increase mAb resolution while maintaining compatibility with all LC instruments • Speed: Shorter analysis times compared to columns packed with fully porous particles of the same size • Lower costs: The robust Poroshell packed bed and 2 µ inlet frit extend column lifetime by helping prevent inlet blockage
AdvanceBio RP-mAb columns are designed to optimize the performance of intact and reduced mAb analysis when analyzing monoclonal antibodies for biopharma discovery, development, and QA/QC applications. Analysis of intact and reduced monoclonal antibodies are critical measurements for characterizing therapeutic proteins and understanding their efficacy and stability. Poor chromatographic separations can result in rework and even compromise the accuracy of the characterization. Based on Poroshell technology with unique engineering for pore size and bonded phases, AdvanceBio RP-mAb columns deliver higher resolution and faster run times to provide accurate, reproducible results. Available in a range of chemistries – SB-C8, C4, and diphenyl. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers . Features: • Improved accuracy: Superficially porous particles (3.5 µm) with wide pores (450Å) increase mAb resolution while maintaining compatibility with all LC instruments • Speed: Shorter analysis times compared to columns packed with fully porous particles of the same size • Lower costs: The robust Poroshell packed bed and 2 µ inlet frit extend column lifetime by helping prevent inlet blockage
AdvanceBio RP-mAb columns are designed to optimize the performance of intact and reduced mAb analysis when analyzing monoclonal antibodies for biopharma discovery, development, and QA/QC applications. Analysis of intact and reduced monoclonal antibodies are critical measurements for characterizing therapeutic proteins and understanding their efficacy and stability. Poor chromatographic separations can result in rework and even compromise the accuracy of the characterization. Based on Poroshell technology with unique engineering for pore size and bonded phases, AdvanceBio RP-mAb columns deliver higher resolution and faster run times to provide accurate, reproducible results. Available in a range of chemistries – SB-C8, C4, and diphenyl. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers . Features: • Improved accuracy: Superficially porous particles (3.5 µm) with wide pores (450Å) increase mAb resolution while maintaining compatibility with all LC instruments • Speed: Shorter analysis times compared to columns packed with fully porous particles of the same size • Lower costs: The robust Poroshell packed bed and 2 µ inlet frit extend column lifetime by helping prevent inlet blockage
HC-C18, 4.6 x 250 mm, 5 u, 2.5 ml solvent, 4.2 ml tube
Eclipse XDB Columns • provide excellent separations over a wide pH range (pH 2 to pH 9), • provide reliable performance under high pressure and flow - a result of Agilent's proprietary ZORBAX silica gel particles, • enable flexible method development with many choices in four bonded phases and a wide range of particle sizes and dimensions.
Eclipse XDB Columns • provide excellent separations over a wide pH range (pH 2 to pH 9), • provide reliable performance under high pressure and flow - a result of Agilent's proprietary ZORBAX silica gel particles, • enable flexible method development with many choices in four bonded phases and a wide range of particle sizes and dimensions.
The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed-phase HPLC. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution. Features • Traditional, fully porous particles available in 1.8, 3.5, and 5 µm sizes. With selected products also available in 7 μm particles for preparative HPLC • ZORBAX Extend-C18 columns offer high efficiency for high pH applications up to pH 11.5
ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series. The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions. The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation. Features • High efficiency and reproducibility with short analysis time • Semi-prep and prep column dimensions • Compatible with organic modifiers and denaturants • Wide usable pH range (3-8)
ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series. The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions. The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation. Features • High efficiency and reproducibility with short analysis time • Semi-prep and prep column dimensions • Compatible with organic modifiers and denaturants • Wide usable pH range (3-8)
Low-cost C18 columns for legacy HPLC applications. Available in moderate and high carbon loads.
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hypersil AA-ODS, 5 µm, 2.1 x 200 mm. 400 bar pressure limit
Hypersil ODS (5021-1845 cartridge holder required (AC))
LiChrosorb HPLC Column. (Use with hardware kit 5021-1845)
LiChrospher 100 RP-8, (use with hardware kit 5021-1845)
LiChrospher column (use with hardware kit (AC) 5021-1845)
Thorough characterization of monoclonal antibodies includes the identification and monitoring of acidic and basic isoforms. The Agilent Bio MAb HPLC columns feature a unique resin specifically designed for high-resolution charge-based separations of monoclonal antibodies. Bio MAb columns are available in 1.7 μm, 3 μm, 5 μm, and 10 μm sizes, providing higher resolution with the smaller particles. The Bio MAb column features a highly uniform, densely packed, weak cation exchange layer. This layer of the Bio MAb column is chemically bonded to the hydrophilic, polymeric coating of the Bio MAb column. Features • A packing support composed of a rigid, spherical, highly cross-linked polystyrene divinylbenzene (PS/DVB) non-porous bead • Particles grafted with a hydrophilic, polymeric layer, virtually eliminating non-specific binding of antibody proteins • A different process is used to layer the weak cation-exchange phase to the particle making it a higher density than the Agilent Bio WCX column particles • Specifically designed for the separation of charge isoforms of monoclonal antibodies
Agilent Bio Ion-Exchange HPLC columns are packed with polymeric, nonporous, ion-exchange particles. Bio Ion-Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins. The Bio IEX family offers strong cation-exchange (SCX), weak cation-exchange (WCX), strong anion-exchange (SAX) and weak anion-exchange (WAX) phases. All Bio Ion-Exchange phases are available in 1.7, 3, 5 and 10 μm non-porous particles sizes. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers . Features • Highly crosslinked and rigid nonporous poly(styrene divinylbenzene) (PS/DVB) particles are grafted with a hydrophilic, polymeric layer, eliminating nonspecific binding • Uniform, densely packed ion-exchange functional groups are chemically bonded to the hydrophilic layer (multiple ion-exchange groups per anchoring) to increase column capacity • Particles, coating and bonding are resistant to high pressures, promoting higher resolution and faster separations • Multiple ion-exchange groups are captured on one anchoring to increase capacity
Agilent Bio Ion-Exchange HPLC columns are packed with polymeric, nonporous, ion-exchange particles. Bio Ion-Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins. The Bio IEX family offers strong cation-exchange (SCX), weak cation-exchange (WCX), strong anion-exchange (SAX) and weak anion-exchange (WAX) phases. All Bio Ion-Exchange phases are available in 1.7, 3, 5 and 10 μm non-porous particles sizes. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers . Features • Highly crosslinked and rigid nonporous poly(styrene divinylbenzene) (PS/DVB) particles are grafted with a hydrophilic, polymeric layer, eliminating nonspecific binding • Uniform, densely packed ion-exchange functional groups are chemically bonded to the hydrophilic layer (multiple ion-exchange groups per anchoring) to increase column capacity • Particles, coating and bonding are resistant to high pressures, promoting higher resolution and faster separations • Multiple ion-exchange groups are captured on one anchoring to increase capacity
Agilent Bio Ion-Exchange HPLC columns are packed with polymeric, nonporous, ion-exchange particles. Bio Ion-Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins. The Bio IEX family offers strong cation-exchange (SCX), weak cation-exchange (WCX), strong anion-exchange (SAX) and weak anion-exchange (WAX) phases. All Bio Ion-Exchange phases are available in 1.7, 3, 5 and 10 μm non-porous particles sizes. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers . Features • Highly crosslinked and rigid nonporous poly(styrene divinylbenzene) (PS/DVB) particles are grafted with a hydrophilic, polymeric layer, eliminating nonspecific binding • Uniform, densely packed ion-exchange functional groups are chemically bonded to the hydrophilic layer (multiple ion-exchange groups per anchoring) to increase column capacity • Particles, coating and bonding are resistant to high pressures, promoting higher resolution and faster separations • Multiple ion-exchange groups are captured on one anchoring to increase capacity
Agilent Bio Ion-Exchange HPLC columns are packed with polymeric, nonporous, ion-exchange particles. Bio Ion-Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins. The Bio IEX family offers strong cation-exchange (SCX), weak cation-exchange (WCX), strong anion-exchange (SAX) and weak anion-exchange (WAX) phases. All Bio Ion-Exchange phases are available in 1.7, 3, 5 and 10 μm non-porous particles sizes. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers . Features • Highly crosslinked and rigid nonporous poly(styrene divinylbenzene) (PS/DVB) particles are grafted with a hydrophilic, polymeric layer, eliminating nonspecific binding • Uniform, densely packed ion-exchange functional groups are chemically bonded to the hydrophilic layer (multiple ion-exchange groups per anchoring) to increase column capacity • Particles, coating and bonding are resistant to high pressures, promoting higher resolution and faster separations • Multiple ion-exchange groups are captured on one anchoring to increase capacity
Bio-Monolith HPLC columns provide high resolution and rapid separations of antibodies (IgG, IgM), plasmid DNA, viruses, phages and other macro biomolecules. The product family offers strong cation exchange, strong and weak anion exchange, and Protein A phases. These columns deliver flow rate-independent separation, speeding up method development time and decreasing costs. Bio-Monolith HPLC columns are compatible with HPLC and preparative LC systems, including Agilent 1100 and 1200 Infinity Series. Features • Polymer-based, monolith HPLC columns designed for macro biomolecule separations • Flow-rate independent separations • No diffusion, no pores and no void volume make transport between mobile and stationary phase very rapid • Monolith disk is 5.2 mm x 4.95 mm (100 μL column volume) with continuous channels, eliminating diffusion mass transfer • Extremely fast separations speed up method development time and decrease costs • locking in method parameters
Bio-Monolith HPLC columns provide high resolution and rapid separations of antibodies (IgG, IgM), plasmid DNA, viruses, phages and other macro biomolecules. The product family offers strong cation exchange, strong and weak anion exchange, and Protein A phases. These columns deliver flow rate-independent separation, speeding up method development time and decreasing costs. Bio-Monolith HPLC columns are compatible with HPLC and preparative LC systems, including Agilent 1100 and 1200 Infinity Series. Features • Polymer-based, monolith HPLC columns designed for macro biomolecule separations • Flow-rate independent separations • No diffusion, no pores and no void volume make transport between mobile and stationary phase very rapid • Monolith disk is 5.2 mm x 4.95 mm (100 μL column volume) with continuous channels, eliminating diffusion mass transfer • Extremely fast separations speed up method development time and decrease costs • locking in method parameters
Bio-Monolith HPLC columns provide high resolution and rapid separations of antibodies (IgG, IgM), plasmid DNA, viruses, phages and other macro biomolecules. The product family offers strong cation exchange, strong and weak anion exchange, and Protein A phases. These columns deliver flow rate-independent separation, speeding up method development time and decreasing costs. Bio-Monolith HPLC columns are compatible with HPLC and preparative LC systems, including Agilent 1100 and 1200 Infinity Series. Features • Polymer-based, monolith HPLC columns designed for macro biomolecule separations • Flow-rate independent separations • No diffusion, no pores and no void volume make transport between mobile and stationary phase very rapid • Monolith disk is 5.2 mm x 4.95 mm (100 μL column volume) with continuous channels, eliminating diffusion mass transfer • Extremely fast separations speed up method development time and decrease costs • locking in method parameters
Bio-Monolith HPLC columns provide high resolution and rapid separations of antibodies (IgG, IgM), plasmid DNA, viruses, phages and other macro biomolecules. The product family offers strong cation exchange, strong and weak anion exchange, and Protein A phases. These columns deliver flow rate-independent separation, speeding up method development time and decreasing costs. Bio-Monolith HPLC columns are compatible with HPLC and preparative LC systems, including Agilent 1100 and 1200 Infinity Series. Features • Polymer-based, monolith HPLC columns designed for macro biomolecule separations • Flow-rate independent separations • No diffusion, no pores and no void volume make transport between mobile and stationary phase very rapid • Monolith disk is 5.2 mm x 4.95 mm (100 μL column volume) with continuous channels, eliminating diffusion mass transfer • Extremely fast separations speed up method development time and decrease costs • locking in method parameters
Please fill out your contact data and your information request, we will contact you as soon as possible with all requested information.
Please fill out your contact data and your information request, we will contact you as soon as possible with all requested information.
