PrepHT, Replacement O-ring, 21.2 mm Cartridge
PLgel MIXED GPC columns offer the most simple and reliable GPC analysis. Every column is rigorously tested to ensure the retention time is directly proportional to molecular weight. Data analysis is easy on any software and chromatograms are easy to read. MIXED columns also eliminate the complexity of stacking individual pore size columns. Adding columns increases resolution, but calibration remains linear.
ZORBAX Aqueous Method Development Kit, includes ZORBAX Bonus-RP, SB-Aq, and SB-C18 columns, 4.6 x 150 mm, 5 µm
High Perf. ZORBAX guard fittings kit-includes low-volume guard holder, inlet end fitting (2), outlet end fitting with integrated column connector, and PEEK fingertight fitting, seals (2/pk)
Includes 4.6 x 75 mm, 3.5 μm columns; one each: XDB-C18, XDB-C8 and XDB-Phenyl phases
Includes 4.6 x 75 mm, 3.5 μm columns; one each: SB-C18, SB-CN and SB-Phenyl phases
• Rugged, high and low pH separations of polypeptides and peptides from pH 2-11.5 • Different selectivity possible at high and low pH • High efficiency and good recovery of hydrophobic peptides at high pH • Ideal for LC/MS with ammonium-hydroxide-modified mobile phase Agilent ZORBAX 300Å Extend-C18 is a wide-pore HPLC column for high efficiency separations of peptides from pH 2-11.5. The unique, bidentate bonded phase provides excellent lifetime and reproducibility at high and low pH. At high pH, retention and selectivity of peptides and polypeptides can change dramatically as a result of changes in charge on molecules. Excellent recoveries of hydrophobic polypeptides have been achieved at room temperature and high pH. LC/MS sensitivity of peptides and polypeptides can also be improved at high pH using a simple ammonium-hydroxide-containing mobile phase.
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations.
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations.
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations. Features • Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins • Exceptional stability and long lifetime at low pH with TFA containing mobile phases • Excellent reproducibility • Available in capillary and nano column dimensions for proteomics and other LC/MS applications
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations. Features • Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins • Exceptional stability and long lifetime at low pH with TFA containing mobile phases • Excellent reproducibility • Available in capillary and nano column dimensions for proteomics and other LC/MS applications
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations. Features • Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins • Exceptional stability and long lifetime at low pH with TFA containing mobile phases • Excellent reproducibility • Available in capillary and nano column dimensions for proteomics and other LC/MS applications
ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations. Features • Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins • Exceptional stability and long lifetime at low pH with TFA containing mobile phases • Excellent reproducibility • Available in capillary and nano column dimensions for proteomics and other LC/MS applications
ZORBAX Ion Exchange columns are available as both Strong Anion-Exchange (SAX) and Strong Cation-Exchange (300SCX) columns. Each column is packed with bonded, 5 µm, spherical silica particles for optimum efficiency in separating and purifying biological macromolecules, including proteins, peptides and polynucleotides. ZORBAX Ion Exchange columns exhibit a high degree of mechanical stability. Therefore, they can be used at high operating flow rates and pressures. The columns are compatible with rapid changes in ionic strength, thus short gradient times and rapid re-equilibration are possible. Features • ZORBAX SAX and 300SCX columns are based on rugged ZORBAX silica • Stable from pH 2–7 • Provide high efficiency, rapid separations • Compatible with organic mobile phase modifiers
AdvanceBio Glycan Mapping columns are designed and manufactured to deliver fast, high resolution, reproducible glycan identification using HILIC chromatography. Two UHPLC configurations are available: 2.7 µm superficially porous, for high resolution and lower backpressure, and 1.8 µm for highest resolution. AdvanceBio Glycan Mapping columns leverage technology that optimizes results for MS and fluorescence detection. They can deliver glycan maps in less than 10 minutes - 40% less time than competing products - and can be ordered with a selection of standards for performance testing and retention mapping of labeled and unlabeled glycans.
AdvanceBio Oligonucleotide columns offer high-quality, high-resolution ion-pair reversed-phase (IP-RP) column chemistry platform that support analytical characterization through purification with seamless method scale-up. To successfully separate trityl-off, deprotected oligonucleotides, HPLC columns need to have high resolving power and be robust enough to withstand high pH and temperature, which provide better separation and denaturing conditions for optimal resolution and oligonucleotide separation. AdvanceBio oligonucleotide columns feature high efficiency, 2.7 and 4 μm superficially porous Poroshell particles. Using technology exclusive to Agilent, we chemically modify the particles to make them exceedingly resistant to high pH mobile phases. We also bond them with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides. In addition, we test every batch of AdvanceBio oligonucleotide media with a resolution standard to ensure consistent performance.AdvanceBio products are designed to deliver consistent, exceptional performance for the complete characterization and bioanalysis of proteins, antibodies, conjugates, new biological entities and biopharmaceuticals.
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