Analytical EC column. NUCLEOSIL® 100-7 C6H5 particle size 7 µm, pore size 100 Å, not endcapped
Analytical EC column. NUCLEOSIL® 100-5 C8 ec particle size 5 µm, pore size 100 Å, endcapped, 9 % C
Analytical EC column. NUCLEOSIL® 100-5 N(CH3)2 particle size 5 µm, pore size 100 Å
Analytical EC column. These HPLC columns are packed with polar aminopropyl modification, based on the original NUCLEOSIL silica gel.
Analytical EC column. NUCLEOSIL® 100-5 OH (Diol) particle size 5 µm, pore size 100 Å. Technical data: · Dihydroxypropyl modiied silica for RP and NP chromatography · Less polar than unmodiied silica, very easily wettable with water · pH stability at 20 °C: 2-8; carbon content 5 %
Analytical EC column. NUCLEOSIL® dimethyl phase (C2) · USP L16 Technical data · Non-endcapped phase for RP and ion-pairing chromatography · pH stability at 20 °C: 2–8; carbon content 3.5 % · Retention times are much shorter than for the other RP phases
Analytical EC column. NUCLEOSIL® SiOH unmodiied silica · USP L3 Technical data · Spherical silica, pH stability 2–8 · Maximum working pressure for the EC columns listed below is 400 bar
Analytical EC column. NUCLEOSIL® 50-5 C18 ec particle size 5 µm, pore size 50 Å, endcapped, 14.5 % C
Analytical EC column NUCLEOSHELL® HILIC zwitterionic phase Core-shell technology for fast and eicient HPLC Ideal for reproducible and stable chromatography of highly polar analytes Very short column equilibration times Technical data Ammonium - sulfonic acid modiied silica; pore size 90 Å, particle size 2.7 μm; carbon content 1.3 %; pH stability 2–8.5; suitable for LC/MS Recommended application Hydrophilic compounds such as polar organic acids and bases, polar natural compounds, nucleosides, oligonucleotides, amino acids, peptides, water-soluble vitamins
Preparative VarioPrep columns. NUCLEODUR® SiOH unmodiied silica for normal phase · USP L3 Key feature: · Totally spherical high purity silica · Pressure stable up to 600 bar · Suitable for analytical and preparative separation of polar and midpolar compounds Technical data: · Unmodiied high purity silica; pore size 110 Å; particle sizes 3 to 50 μm; pore volume 0.9 mL/g; surface area (BET) 340 m2/g; pH stability 2–8; metal content < 10 ppm Recommended application · Polar and midpolar compounds under normal phase conditions
Preparative VarioPrep columns. NUCLEODUR® C8 Gravity nonpolar high density phase · USP L7 Key feature: · Suitable for LC/MS and HPLC at pH extremes (pH 1–11) · Superior base deactivation · Ideal for method development Technical data: · Available as octadecyl (C18) and octyl (C8 ), multi-endcapped · Pore size 110 Å; particle sizes 7, 10, 12 and 16 μm particles for preparative purposes on request · Carbon content 18 % for C18, 11 % for C8 Recommended application: · Overall sophisticated analytical separations · Compound classes separated include pharmaceuticals, e.g., analgesics, anti-inlammatory drugs, antidepressants; herbicides; phytopharmaceuticals; immunosuppressants
Analytical EC column, phase for highly aqueous eluents. Stable in 100 % aqueous mobile phase systems Interesting polar selectivity features Excellent base deactivation; suitable for LC/MS due to low bleeding characteristics Technical characteristics: Special phase with polar endcapping; pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm (7 and 10 μm particles for preparative purposes on request); carbon content 14 %; pH stability 1–9 Recommended application: Analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agents, organic acids USP L1
EC HPLC column NUCLEODUR® C18 ec ∙ C8 ec nonpolar phases for routine analysis Key features: • Ideal and reliable standard RP phase for daily routine analysis and up-scaling for preparative HPLC • Medium density octadecyl (C18) and octyl (C8) modification with exhaustive endcapping • Wide range of application areas Technical characteristics: Pore size 110 Å; particle sizes 3 μm and 5 μm; 7 μm, 10 μm, 12 μm, 16 μm, 20 μm, 30 μm and 50 μm for preparative separations; carbon content 17.5 % for C18, 10.5 % for C8 pH stability 1–9, high reproducibility from lot to lot Recommended application: Basic, neutral or acidic drugs, derivatized amino acids, pesticides fat-soluble vitamins, aldehydes and ketones, phenolic compounds USP L1 (C18) / L7 (C8)
EC HPLC column NUCLEODUR® C18 ec ∙ C8 ec nonpolar phases for routine analysis Key features: • Ideal and reliable standard RP phase for daily routine analysis and up-scaling for preparative HPLC • Medium density octadecyl (C18) and octyl (C8) modification with exhaustive endcapping • Wide range of application areas Technical characteristics: Pore size 110 Å; particle sizes 3 μm and 5 μm; 7 μm, 10 μm, 12 μm, 16 μm, 20 μm, 30 μm and 50 μm for preparative separations; carbon content 17.5 % for C18, 10.5 % for C8 pH stability 1–9, high reproducibility from lot to lot Recommended application: Basic, neutral or acidic drugs, derivatized amino acids, pesticides fat-soluble vitamins, aldehydes and ketones, phenolic compounds USP L1 (C18) / L7 (C8)
Analytical EC column, zwitterionic phase. Ideal for reproducible and stable chromatography of highly polar analytes • Suitable for analytical and preparative applications as well as LC/MS • Very short column conditioning period Technical characteristics: Ammonium - sulfonic acid modified silica; pore size 110 Å; particle sizes 1.8, 3 and 5 μm; carbon content 7 %; pH stability 2–8.5 Recommended application: Hydrophilic compounds such as organic polar acids and bases, polar natural compounds, nucleosides, oligonucleotides, amino acids, peptides, water soluble vitamins
EC HPLC column NUCLEODUR® C18 ec ∙ C8 ec nonpolar phases for routine analysis Key features: • Ideal and reliable standard RP phase for daily routine analysis and up-scaling for preparative HPLC • Medium density octadecyl (C18) and octyl (C8) modification with exhaustive endcapping • Wide range of application areas Technical characteristics: Pore size 110 Å; particle sizes 3 μm and 5 μm; 7 μm, 10 μm, 12 μm, 16 μm, 20 μm, 30 μm and 50 μm for preparative separations; carbon content 17.5 % for C18, 10.5 % for C8 pH stability 1–9, high reproducibility from lot to lot Recommended application: Basic, neutral or acidic drugs, derivatized amino acids, pesticides fat-soluble vitamins, aldehydes and ketones, phenolic compounds USP L1 (C18) / L7 (C8)
EC HPLC column NUCLEODUR® C18 ec ∙ C8 ec nonpolar phases for routine analysis Key features: • Ideal and reliable standard RP phase for daily routine analysis and up-scaling for preparative HPLC • Medium density octadecyl (C18) and octyl (C8) modification with exhaustive endcapping • Wide range of application areas Technical characteristics: Pore size 110 Å; particle sizes 3 μm and 5 μm; 7 μm, 10 μm, 12 μm, 16 μm, 20 μm, 30 μm and 50 μm for preparative separations; carbon content 17.5 % for C18, 10.5 % for C8 pH stability 1–9, high reproducibility from lot to lot Recommended application: Basic, neutral or acidic drugs, derivatized amino acids, pesticides fat-soluble vitamins, aldehydes and ketones, phenolic compounds USP L1 (C18) / L7 (C8)
Analytical EC column, hydrophobic pentafluorophenyl phase. Hydrophobic phase with alternative selectivity in comparison to classical C18 modifications Separation principle based on 4 retention mechanisms (polar interactions (H bonds), dipole-dipole, π-π, and hydrophobic interactions)Suitable for LC/MS due to low bleeding characteristics Technical characteristics: Phase with pentafluorophenyl-propyl modification and multi-endcapping; pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm; carbon content 8 %; pH stability 1–9 Recommended application: Aromatic and unsaturated compounds, phenols, halogen compounds, isomers, polar compounds like pharmaceuticals, antibiotics; strong retention of basic compounds USP L43
EC HPLC column NUCLEODUR® C18 ec ∙ C8 ec nonpolar phases for routine analysis Key features: • Ideal and reliable standard RP phase for daily routine analysis and up-scaling for preparative HPLC • Medium density octadecyl (C18) and octyl (C8) modification with exhaustive endcapping • Wide range of application areas Technical characteristics: Pore size 110 Å; particle sizes 3 μm and 5 μm; 7 μm, 10 μm, 12 μm, 16 μm, 20 μm, 30 μm and 50 μm for preparative separations; carbon content 17.5 % for C18, 10.5 % for C8 pH stability 1–9, high reproducibility from lot to lot Recommended application: Basic, neutral or acidic drugs, derivatized amino acids, pesticides fat-soluble vitamins, aldehydes and ketones, phenolic compounds USP L1 (C18) / L7 (C8)
EC HPLC column NUCLEODUR® NH2 / NH2-RP amino-modified high purity silica Key features: • Multi-mode columns (for RP, NP and IC) • Stable against hydrolysis at low pH (working range pH 2–8), 100 % stable in water; suitable for LC/MS • Widens scope of analytical HPLC into the polar range Technical characteristics: Aminopropyl modified high purity silica; pore size 110 Å; particle sizes 3, 5 and 7 μm; carbon content 2.5 %; not endcapped Recommended application: Polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditions USP L8 • Normal phase chromatography (NP) with hexane, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticides • Reversed phase chromatography (RP) of polar compounds in aqueous-organic eluent systems • Ion exchange chromatography of anions and organic acids using conventional buffers and organic modifiers
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