With Thermo Scientific™ Acclaim™ E LC Columns, Nitroaromatics and Nitramines by HPLC, you can achieve baseline resolution of all 14 explosives listed in EPA SW-846 Method 8330. Their unique column chemistries provide excellent resolution with complementary selectivities. Use the E1 column (sold separately) as a direct replacement for ODS columns for primary analysis. Then use the E2 column as a primary or confirmatory column. Simply change the separation conditions for the E2 column to cover a wider range of applications, including the Analysis of explosives beyond US EPA Method 8330. Outstanding performance for explosives analysis • Repeatable performance • Simple isocratic elution conditions • Unique sensitivity for the separation of additional nitroaromatic molecules • Robust columns with good reproducibility over multiple batches Information about the Acclaim family of HPLC columns for specialty applications • Designed, manufactured and tested to meet the requirements of specific applications • The columns offer excellent resolution with easy operation • The consistency of the separations has proven itself in practice • Ideal selectivity for the separation of specific target analytes • Excellent peak shapes • Performance tested to ensure high quality and reliability
Benefit from high-performance analysis of hydrophobic amines and acids with suppressed conductivity detection with high-efficiency, silica-based Thermo Scientific™ Dionex™ IonPac™ NS2 Reversed Phase Columns. This column is designed for mobile phase ion chromatography (MPIC) using eluents , which contain trifluoroacetic acid (TFA), heptafluorobutyric acid (HFBA), hexanesulfonic acid (HSA), octanesulfonic acid (OSA), tetrapropylammonium (TPA) borate, tetrabutylammonium (TBA), borate and sodium borate. The Dionex IonPac NS2 column is also suitable for conventional LC -Applications (reverse phase liquid chromatography). MPIC is a method in which an ion-pairing agent in the eluent improves retention for charged analytes in a hydrophobic column and is then removed from the suppressor to enable selective, sensitive detection of the analytes. For the analysis of cations, heptafluorobutyric acid is one such agent suitable for suppression. Main functions of the column: • Excellent resolution • Excellent peak shape • High effectiveness • Robust fixed bed Eluent guidelines Anions typically use 5 to 10 mM sodium borate, 0.5 to 5 mM TPA or TBA borate with a pH of 8 to 9, prepared from boric acid and the corresponding hydroxide. When performing gradient elution with a Thermo Scientific™ Dionex™ ASRS™ Anion Self-Regenerating Suppressor, we recommend keeping the sodium/TBA concentration constant and varying the organic solvent. Cations typically use 0.5 to 10 mM TFA, HFBA, HSA or OSA. When performing gradient elution with a Thermo Scientific™ Dionex™ ASRS™ Cation Self-Regenerating Suppressor, we recommend keeping the acid concentration constant and varying the organic solvent. The solvent composition is limited due to the solvent compatibility of the suppressor. Before shutting down the system, flush all solvent from the suppressor with DI water. Please refer to the suppressor manuals for detailed solvent compatibility instructions and limits. Instruction manual for the suppressor • The 4 mm Thermo Scientific™ Dionex™ AERS™ Electrolytically Regenerated Anion Suppressors, Dionex ASRS Suppressors, Thermo Scientific™ Dionex™ CERS™ Electrolytically Regenerated Cation Suppressors and Dionex CSRS Suppressors are compatible with ≤40% acetonitrile and require operation with foreign water . • Thermo Scientific™ Dionex™ AMMS™ Anion MicroMembrane™ Suppressors are compatible with all solvents containing 5-50 mN sulfuric acid at 2-10 mL/min. • Thermo Scientific™ Dionex™ CMMS™ Cation MicroMembrane™ Suppressors are compatible with all solvents containing 20-100 mN tetrabutylammonium hydroxide at 2-100 mL/min. • For detailed solvent compatibility instructions and limits, see the suppressor manuals.
Achieve superior reversed phase oligonucleotide separations using the Thermo Scientific™ DNAPac™ RP HPLC columns. The unique chemistry is designed for analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC-UV or LC-MS. The column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions. In addition, the wide pore size of the resin provides excellent separation of large double-stranded nucleic acids up to 10k base pairs. High Performance Reversed-Phase LC Columns The DNAPac RP column is based on spherical wide-pore 4μm polymer resin. The phase is highly stable to extremes of pH (0-14), and temperature (5-110°C), and operates with mobile phases compatible with direct mass spectrometry. The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments. DNAPac RP reversed phase (RP) column is specifically designed for separation of oligonucleotides and DNA/RNA fragments. The stationary phase is fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of large nucleic acids with low carry-over. Columns designed for reversed phase oligonucleotide LC and LC/MS separations • Designed for ion-pair reversed-phase (IP-RP) separations of both oligonucleotides and double-stranded nucleic acids • High resolution and high throughput • Excellent MS compatibility • Wide operating pH range (0-14) and high temperature stability (up to 110°C)
Get maximum loading for your scale up from analytical to a prep LC method with Thermo Scientific™ Hypersil™ PREP HS C8 reversed phase preparative HPLC column. With similar selectivity to C18 columns and predictable elution order, C8 columns are ideal for analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention. The lower hydrophobicity allows compounds to elute more quickly, yielding faster separations. By providing high surface area particles with a 100Å pore size, and a variety of dimensions including an option for XtendedLife hardware, which improves column lifetime by maintaining a uniform packed bed. Similar selectivity, less retention than C18 columns • Successful retention and separation of moderately hydrophobic analytes. • Ideal for fatty acids, which are too hydrophobic for analysis using C18 columns. • • C8 (USP L7) selectivity - high surface area particle with 100Å pore size. • pH stable from 2 to 8. • Maximum temperature of 60°C. Faster separations • Higher analytical throughput compared to C18 alkyl chain chemistry. • Quicker compound elution due to reduced hydrophobic interactions. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. These preparative guard columns require the use of a preparative guard holder.
Manufactured with a proprietary process and subjected to stringent quality control procedures,Thermo Scientific™ HyperREZ™ XP Carbohydrate Pb2+ LC Columns are used for the appropriate counter-ion to meet application requirements. Each column is individually tested to ensure column-to-column reproducibility. Polymer-based columns for carbohydrate analysis offer benefits to the chromatographer including temperature and pH stability, as well as enhanced solvent choices. • Designed for the determination of carbohydrates, saccharides, organic acids and alcohols • Efficient and reproducible monodisperse particles • Stable for long column lifetimes even at low pH and high temperatures
Manufactured with a proprietary process and subjected to stringent quality control procedures, Thermo Scientific™ HyperREZ™ XP Carbohydrate Ca2+ LC Columns are used for the appropriate counter-ion to meet application requirements. Each column is individually tested to ensure column-to-column reproducibility. Polymer-based columns for carbohydrate analysis offer benefits to the chromatographer including temperature and pH stability, as well as enhanced solvent choices. • Designed for the determination of carbohydrates, saccharides, organic acids and alcohols • Efficient and reproducible monodisperse particles • Stable for long column lifetimes even at low pH and high temperatures
Available in H+, Ca+2, Pb+2, and Na+ forms, Thermo Scientific™ HyperREZ™ XP Carbohydrate Na+ LC Columns are the appropriate counter-ion to meet application requirements. Polymer-based columns for carbohydrate analysis offer benefits to the chromatographer including temperature and pH stability, as well as enhanced solvent choices. • Designed for the determination of carbohydrates, saccharides, organic acids and alcohols • Efficient and reproducible monodisperse particles • Stable for long column lifetimes even at low pH and high temperatures
Simultaneously separate pharmaceutical drug substances and their counterions with high-efficiency Thermo Scientific™ Acclaim™ Trinity P1 LC Columns. A unique trimodal surface chemistry provides concurrent reversed-phase, cation-exchange and anion-exchange functionalities for maximum flexibility in method development. Easily optimize selectivity by adjusting mobile phase buffer concentration, pH and solvent content—either jointly or independently—to accelerate chromatographic separations and increase productivity. The Acclaim Trinity P1 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical 3μm silica particles, coated with charged nanopolymer beads. The unique surface chemistry includes an inner-pore area modified with an organic layer that provides both reversed-phase and anion-exchange properties. The outer-pore surface, conversely, is modified with cation-exchange functionality for unparalleled chromatographic performance. Superior Performance for Pharmaceutical Applications Ideal selectivity for simultaneous separation of Active Pharmaceutical Ingredients (API) and counterions (basic, neutral and acidic analytes) Effective retention of hydrophilic ionic and ionizable analytes without ion-pairing reagents Distinctive spatial separation of the anion-exchange and cation-exchange regions Flexible method development with each retention mechanism controlled independently Low column bleed; compatible with mass spectrometry (MS), charged aerosol detection (CAD) and evaporative light scattering detectors (ELSD) Available in 2.1 and 3.0mm diameters and 50, 100 and 150mm lengths Complement the Acclaim Trinity P1 column with the Acclaim Trinity P2 column to provide a total solution for pharmaceutical counterion analysis by HPLC. Use the Acclaim Trinity P2 column to separate counterions, including monovalent and divalent cations or anions.
Separate challenging peptide mapping samples using Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC columns. These columns feature easy connectivity, high reproducibilty, and excellent separations. Our Neo columns provide 1500 bar pressure capability, improved column-to-column consistency, and increased efficiency. The column media is manufactured and selected to exacting standards and packed at high pressure, resulting in enhanced peak symmetry, resolution, and column-to-column reproducibility that allows you to obtain greater sample coverage and sample insights. The Click-and-Spray format of EASY-Spray columns enables perfect connections to our mass spectrometers every time. Benefits of PepMap Neo Columns • Brilliant Connectivity — EASY-Spray™ columns click into position with ease. • Brilliant Reproducibility — Precise manufacturing protocols for packing media improve column-to-column consistency for reproducible analysis every day. • Brilliant Separation — Ultra-high pressure packing increases column efficiency, allowing you to run at up to 1500 bar for enhanced peak symmetry and resolution, providing higher sample capacity for more sample insights.
Ensure robust nano and capillary flow into your LC-MS using Thermo Scientific™ EASY-Spray™ HPLC Columns. The integrated column/emitter design eliminates dead volume and is temperature-controlled for maximum reliability and performance. Rigorously tested to ensure maximum quality, these columns deliver maximum simplicity and ease-of-use. The capillary flow HPLC columns provide sensitive protein, peptide, and monoclonal antibody (mAb) separation. They give proteomics researchers more than ever before: more throughput, more sensitivity, more separation power and more ease of use. The unique EASY-Spray design provides uncompromised performance and ease-of-use in nano and capillary LC-MS proteomics applications. Features include the following: • Precision positioned glass emitter • Integrated precision designed union • Integrated temperature control
Scale up your analytical method using Thermo Scientific™ Hypersil™ PREP ODS C18 reversed phase preparative HPLC Columns. These columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. This C18 column is a general purpose column used in a broad range of applications, perfect for routine workflows. For Routine Applications • C18 (USP L1) selectivity. • pH stable from 2 to 8. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
The Thermo Scientific™ NativePac OBE-1 Column is a Size Exclusion Chromatography (SEC) column designed for rapid exchange of non-volatile buffer components with a volatile buffer for Native-MS analysis of biomacromolecules. The small pores of the SEC stationary phase maximize separation of the non-volatile components from the sample of interest while the short column length minimizes analysis times to less than 2 minutes. A narrow column ID enables the use of low flow rates for high sensitivity detection and maximizes up time by minimizing buffer consumption.
ACCLAIM TRINITY XL P1 150X25 MM PREP COLUMN
Acclaim™ Phenyl-1 Reversed Phase LC Columns offer high selectivity of aromatic compounds. The specially developed bonded phase shows a higher π-π interaction than other phenyl phases and offers improved aromatic selectivity and higher hydrophobic retention. These columns are suitable is ideal for the analysis of aromatic analytes such as glucocorticosteroids, estrogens, fat-soluble vitamins and phospholipids. High aromatic selectivity for the separation of aromatic compounds • High hydrophobic retention • Compatibility with highly aqueous mobile phases • High efficiency and robust packaging • Extremely low column bleed, fully compatible with MS • Stable in the pH range from 2 to 8 • Available in particle sizes of 3 and 5.0 μm, with diameters of 4.6, 3.0 and 2.1 mm and an average pore diameter of 120 Å
Achieve baseline separation of carbamate pesticides (N-methylcarbamate and N-methylcarbamoyloxime pesticides) specified in U.S. EPA Method 531.2 by using Thermo Scientific™ Acclaim™ Carbamate LC Columns. These high-efficiency columns are compatible with both binary (methanol/water) and ternary (acetonitrile/methanol/water) mobile phase gradients for greater flexibility. Use these extremely low bleed columns with either LC/post-column derivatization/fluorescence or LC/MS detection for the ultimate sensitivity. Multiple Column Formats Meet Your Specific Analysis Needs • 5μm, 4.6 × 250mm Column: Sets the standard for the highest resolution with post-column reaction (PCR) and fluorescence detection (FLD) • 3μm, 4.6×150mm Column: Saves time and increases sample throughput • 3μm, 3.0×150mm Column: Saves time and reduces solvent consumption • 3μm, 2.1×150mm Column: Suited for LC/MS, to achieve the lowest possible detection limits • Two RSLC, 2.2μm Columns (2.1×100mm and 2.1×150mm): Provide high-resolution, high-throughput separations
Ensure robust nano and capillary flow into your LC-MS using Thermo Scientific™ EASY-Spray™ HPLC Columns. The integrated column/emitter design eliminates dead volume and is temperature-controlled for maximum reliability and performance. Rigorously tested to ensure maximum quality, these columns deliver maximum simplicity and ease-of-use. The capillary flow HPLC columns provide sensitive protein, peptide, and monoclonal antibody (mAb) separation. They give proteomics researchers more than ever before: more throughput, more sensitivity, more separation power and more ease of use. The unique EASY-Spray design provides uncompromised performance and ease-of-use in nano and capillary LC-MS proteomics applications. Features include the following: • Precision positioned glass emitter • Integrated precision designed union • Integrated temperature control
Ensure robust nano and capillary flow into your LC-MS using Thermo Scientific™ EASY-Spray™ HPLC Columns. The integrated column/emitter design eliminates dead volume and is temperature-controlled for maximum reliability and performance. Rigorously tested to ensure maximum quality, these columns deliver maximum simplicity and ease-of-use. The capillary flow HPLC columns provide sensitive protein, peptide, and monoclonal antibody (mAb) separation. They give proteomics researchers more than ever before: more throughput, more sensitivity, more separation power and more ease of use. The unique EASY-Spray design provides uncompromised performance and ease-of-use in nano and capillary LC-MS proteomics applications. Features include the following: • Precision positioned glass emitter • Integrated precision designed union • Integrated temperature control
Thermo Scientific™ Acclaim™ HILIC-10 LC Columns separate highly hydrophilic molecules using hydrophilic interaction liquid chromatography. These columns enable the retention and separation of highly polar molecules that are not retained by reversed-phase chromatography. The high-purity, Spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry allows the use of 5 to 20% of an aqueous mobile phase while maintaining affinity for polar analytes. Sample throughput is increased without the need for non-aqueous To evaporate or reconstitute samples dissolved in solvents. Robust and reproducible separations of strongly polar compounds: • Unique selectivity, complementing reversed phase columns • Improved sensitivity in MS detection • Hydrolytically stable • Robust column packaging • Wide range of applications including separation of hydrophilic drugs and their metabolites Acclaim HILIC-10 LC columns are available in a 3 μm particle size and a variety of column formats: 4.6 × 150 mm, for routine, high-throughput analysis; 3.0 × 150 mm, for high throughput analysis and reduced solvent consumption; 2.1 × 150 mm, for LC-MS analysis.
For separations of hydrophobic, structurally related isomers, Thermo Scientific™ Acclaim™ C30 LC reversed-phase columns provide superior results. The covalent modification of C30 alkyl silane on ultrapure, spherical, porous silica gel gives these columns high shape selectivity. They are fully compatible with various aqueous buffers, useful for a broad application range of analytes, and offer more flexibility in method development. High Shape Selectivity for the Separation of Structurally Related Isomers • Unique selectivity complementary to other reversed-phase columns • High efficiency and rugged packing • Extremely low bleed, fully compatible with MS • Stable between pH 2 and 8 • Available in 3 and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 200Å
Maintain exceptional peak shape and resolution for your scale up purification needs using Thermo Scientific™ Hypersil GOLD™ C18 preparative HPLC columns. Our columns come in a variety of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. Hypersil GOLD columns give you confidence when scaling up from your analytical method. Novel Bonding • Proprietary functionalization and end-capping. • C18 (USP L1) selectivity. pH Stability • Robust performance at high pH. • Excellent column stability at low pH. Superior Performance • Highly symmetrical peak shapes. • Improved speed of analysis, efficiency, and sensitivity. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder. Customer Specific Orders • If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in a preparative scale not shown or would like to discuss a specific column dimension, we would be happy to discuss your requirements. • Please contact your local sales representative for Customer Specific Orders. • Custom built columns are not eligible for returns.
Separate challenging peptide mapping samples with Thermo Scientific™ Double nanoViper™ PepMap™ Neo UHPLC columns. These columns feature easy connectivity, high reproducibilty, and excellent separations. Our Neo columns are packed to higher pressure and provide 1500 bar pressure capability, improved column-to-column consistency, and increased efficiency. The column media is manufactured and selected to exacting standards and packed at high pressure, resulting in enhanced peak symmetry, resolution, and column-to-column reproducibility that allows you to obtain greater sample coverage and sample insights. Benefits of PepMap Neo Columns • Brilliant Connectivity — Double nanoViper™ columns connect with ease. • Brilliant Reproducibility — Precise manufacturing protocols for packing media improve column-to-column consistency for reproducible analysis every day. • Brilliant Separation — Ultra-high pressure packing increases column efficiency, allowing you to run at up to 1500 bar for enhanced peak symmetry and resolution, providing higher sample capacity for more sample insights.
Thermo Scientific ™ Acclaim™ HAA is a silica-based application-specific column for high resolution and high throughput trace analysis of haloacetic acids (HAAs) in water samples by LC-MS. 2. Main features • Excellent resolution • Fast analysis • Ideal selectivity • LC-MS compatibility 3. Physical data • Column Chemistry: Proprietary mixed-mode • Silica Substrate: Spherical, high-purity, porous
Use Thermo Scientific™ Acclaim™ Carbonyl C18 LC Columns for the separation of dinitrophenylhydrazine (DNPH) derivatives of aldehydes and ketones with excellent resolution and high throughput. This silica-based reversed-phase column provides excellent selectivity and baseline resolution of DNPH derivatives , which are regulated by various official methods, including US EPA 554, EPA 8315, EPA 1667, EPA TO-11 and CARB 1004. Outstanding performance in demanding aldehyde and ketone analyses • Highly efficient separations enable excellent UHPLC performance • Robust columns with good reproducibility over multiple batches • Proven robust methods A variety of column dimensions to meet your analysis needs • Particle sizes: 2.2, 3 and 5 µm; Smaller particle sizes ensure high peak efficiency for quick analysis • Diameter: 2.1, 3.0 and 4.6 mm; Small diameter columns (2.1 and 3.0 mm) use less solvent and produce less chemical waste • Lengths: 100, 150 and 250 mm For high throughput applications, use the 2.1 × 100 mm column format combined with high linear speed. If high resolution performance is required, use a longer column (2.1 × 150 mm) at an optimal linear speed to achieve even better resolution.
Easily scale up your analytical method using Thermo Scientific™ Hypersil™ PREP BDS Si preparative HPLC columns which are packed with a base deactivated silica for normal phase chromatography of nonpolar and moderately polar organic compounds.These preparative HPLC columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Normal phase chromatography • Base deactivated silica to separate nonpolar and moderately polar organic compounds. • USP L3. • pH stable from 2 to 7. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
Easily scale up your analytical method and achieve maximum retention and loading using Thermo Scientific™ Hypersil™ PREP HS Si preparative HPLC columns. Packed with a bare silica, the high surface area particles with a 100Å pore size are made for normal phase chromatography of nonpolar and moderately polar organic compounds. These preparative HPLC columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Normal phase chromatography • Silica to separate nonpolar and moderately polar organic compounds. • USP L3 - high surface area particle with 100Å pore size. • pH stable from 2 to .7 • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. These preparative guard columns require the use of a preparative guard holder.
Get a high-performance, reversed-phase columns for aminoglycoside analysis. The robust and rugged Thermo Scientific™ Acclaim™ AmG C18 HPLC column provides exceptional tolerance required for aminoglycosides ion-pairing chromatographic separations. These silica-based C18 columns are optimized for various aminoglycoside antibiotic analyses, including drug purity characterization and quantification, therapeutic drug monitoring, and residual control testing in different matrices. These columns are stable at low pH, high temperature, organic solvents, and aqueous mobile phases. Highly Stable for Aminoglycoside Analyses The Acclaim AmG C18 column is specifically designed for ion-pairing reversed-phase HPLC (IP-RPLC) analysis of aminoglycoside antibiotics using volatile perfluorinated carboxylic acids as the ion-pairing reagent, such as 100 mM trifluoroacetic acid (TFA). The Acclaim AmG C18 stationary phase is based on a proprietary C18 bonding technology that provides extremely high stability towards acidic conditions. This specialty column exhibits excellent selectivity and high resolution for the aminoglycoside antibiotics analysis. In addition, it is easy to use because simple aqueous mobile phase (e.g. 100 mM TFA) without organic solvent and pH adjusting buffer is sufficient in most cases. Excellent selectivity for aminoglycosides Superior tolerance towards acidic conditions High efficiency and throughput Ease of use The column can be used with UV, CAD, ELSD detectors
HYPERSIL GOLD SILICA 5UM 250X10MMPREP COLUMN
HYPERSIL GOLD SI 5UM 250X20MMPREP COLUMN
Maintain the sensitivity you need for your purification needs using Thermo Scientific™ Hypersil GOLD™ C8 preparative LC Columns. Best for scale up from analytical to prep LC method and ideal for analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention. The lower hydrophobicity allows compounds to elute more quickly, yielding faster separations. Benefit from similar selectivity to C18 columns with a predictable elution order. Our columns come in a variety of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Similar selectivity, less retention than C18 columns • Successful retention and separation of moderately hydrophobic analytes. • Best for fatty acids, which are too hydrophobic for analysis using C18 columns. • C8 (USP L7) selectivity. Faster Separations • Higher analytical throughput compared to C18 alkyl chain chemistry. • Faster compound elution due to reduced hydrophobic interactions. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.
HYPERSIL GOLD PFP 5UM 150 X 4MM CC
Hypersil PREP HS phenyl chemistry with high loading capacity and fully scalable from analytical to prep, suitable for all USP-L11 applications. A high surface area particle with a 100Å pore size and a phenyl bonded phase gives the stationary phase moderate hydrophobicity, making it ideal for the separation of analyte mixtures with varying polarity and aromaticity perfect for small molecule and drug purification needs. Unique Selectivity • Enhanced selectivity for aromatic and moderately polar analytes. • Selectivity complementary to that provided by alkyl chain phases. • Moderate hydrophobicity compared to C18. • Phenyl (USP L11) selectivity - high surface area particle with 100Å pore size. • pH stable from 2 to 8. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. These preparative guard columns require the use of a preparative guard holder.
Thermo Scientific™ Acclaim™ Vanquish™ C18 UHPLC Columns ensure improved separation performance and speed as well as higher throughput. These reversed phase columns were developed in conjunction with the Vanquish UHPLC system to take advantage of the system's expanded printing capabilities and robustness. The result is a new level of separation, speed and throughput that solves analytical challenges in liquid chromatography (LC) and LC-MS analyses. For powerful separations The expanded offering of Thermo Scientific™ Accucore™ Vanquish™ and Acclaim VANQUISH UHPLC columns provides a greater range of selectivity and format options to meet the diverse needs of UHPLC applications. They offer: • Better separations: high efficiency enables the separation of very complex mixtures • Further results: High efficiency at high flow rates • Easier Interaction: Seamless workflow solution for easy and hassle-free disconnects • Advanced features: Wide range of selectivities and formats
Achieve even greater power of separation, speed, and throughput of polar and nonpolar compounds in a single run using Thermo Scientific™ Acclaim™ VANQUISH™ PA2 UHPLC columns. These reversed-phase columns were developed in conjunction with the Vanquish UHPLC system to take advantage of the system's extended pressure capabilities and robustness. The result is a new level of separation, speed and throughput that solve analytical challenges in liquid chromatography (LC) and LC-MS analyses. These Polar Advantage II columns provide selectivity that is complementary to conventional C18 columns. Delivering Powerful Separations The expanded range of Thermo Scientific™ Accucore™ Vanquish™ and Acclaim VANQUISH UHPLC columns offer a wider range of selectivity and format options to satisfy different UHPLC application requirements. They deliver: Better Separations: high efficiency enables separation of very complex mixture More Results: High efficiency at high flow rates Easier Interactions: Seamless workflow solution, for simple and easy separations Wider Capabilities: Wide range of column selectivities and formats
Separate water-soluble polymers and oligomers with Thermo Scientific™ Acclaim™ SEC-1000 Size Exclusion HPLC Columns. These resin-based, high-performance, size-exclusion chromatography columns cover molecular weight range: 7μm, 1000Å. The combination of a proprietary mono-dispersed multi-pore hydrophilic resin and small particle size provides high-resolution and good linearity of the calibration curve with no inflection points for accurate, reliable analysis of a variety of water-soluble polymers. The Acclaim SEC-1000 LC column is calibrated from 1,000 to 1,000,000 Daltons and can characterize high molecular weight polymers. High-Performance Analysis of Water-Soluble Polymers High-resolution analysis at reduced solvent consumption in available 4.6 x 300mm dimension Stable surface bonding leads to low column bleed and enhances compatibility with UV, RI, MS, ELSD and Thermo Scientific Dionex™ Corona™ Charged Aerosol Detectors
Preperative Hypersil GOLD aQ polar endcapped C18 columns are stable in 100% aqueous mobile phase and provide enhanced retention and resolution of polar analytes. The HPLC columns come in a wide range of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Alternative selectivity with polar endcapped C18 stationary phase • Polar functional group in endcap provides further interaction mechanism for separation of polar compounds. • Yields superior retention of polar compounds. • C18 (USP L1) selectivity. Stability in highly aqueous mobile phases • Applicable in 100% aqueous mobile phases. • No loss of performance or stability. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.
Separate water-soluble polymers and oligomers with Thermo Scientific™ Acclaim™ SEC-300 Size Exclusion Chromatography HPLC Columns. These resin-based, high-performance, size-exclusion chromatography columns cover molecular weight range: 5μm, 300Å. The combination of a proprietary mono-dispersed multi-pore hydrophilic resin and small particle size provides high-resolution and good linearity of the calibration curve with no inflection points for accurate, reliable analysis of a variety of water-soluble polymers. The Acclaim SEC-300 column is calibrated from 100 to 50,000 Daltons and offers good resolution for lower molecular weight polymers or oligomers. High-Performance Analysis of Water-Soluble Polymers • High-resolution analysis at reduced solvent consumption in available 4.6 x 300mm dimension • Stable surface bonding leads to low column bleed and enhances compatibility with UV, RI, MS, ELSD and Thermo Scientific Dionex™ Corona™ Charged Aerosol Detectors
Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific™ Acclaim™ Trinity P2 LC Columns. The innovative, mixed-mode column technology provides hydrophilic interaction, HILIC, anion-exchange, and cation-exchange functionalities for flexible method development. Use the Acclaim Trinity P2 LC column to complement the Acclaim Trinity P1 LC column, which simultaneously separates pharmaceutical drug substances and their counterions, to provide a total solution for pharmaceutical counterion analysis by HPLC. The Acclaim Trinity P2 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical silica particles, coated with charged nanopolymer particles. The unique surface chemistry includes an inner-pore area of the silica bead modified with a covalently bonded organic layer that provides cation-exchange retention. The outer-pore surface is modified with anion-exchange nano-polymer beads. This chemistry ensures spatial separation of the anion-exchange and cation-exchange regions. High Selectivity for Counterion Analysis • Low column bleed; compatible with mass spectrometry (MS) and charged aerosol detection (CAD) • Hydrolytically stable • High efficiency • Available in 3.0μm particle size and 2.1 and 3.0mm diameters, in 50 and 100mm lengths
HYPERSIL GOLD PFP 5UM PREP COLUMN
Attain faster separations for hydrophobic analytes when you scale up using Thermo Scientific™ Hypersil GOLD™ C4 reversed phase preparative HPLC Columns, for your purification needs. These C4 columns provide excellent sensitivity and high efficiency, highlighting a lower hydrophobicity than C8 or C18 columns. These columns come in a variety of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Similar selectivity, less retention than C18 or C8 columns • Outstanding retention and separation of very hydrophobic analytes with this short alkyl chain length, low hydrophobicity column. • Best for analysis of hydrophobic polypeptides and proteins. • C4 (USP L26) selectivity. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d.
HYPERSIL GOLD CN 5UM PREP Column
HYPERSIL GOLD SAX 5UM 150X20MM PREP Column
Use Thermo Scientific™ Hypersil™ PREP Si preparative HPLC columns to scale up your normal phase chromatography analytical method. The silica chemistry separates nonpolar and moderately polar organic compounds. These preparative HPLC columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Normal phase chromatography Silica to separate nonpolar and moderately polar organic compounds. USP L3. pH stable from 2 to 7. Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. Standard threaded hardware for column i.d. < 40mm. Standard flange hardware for column i.d. ≥ 40mm. Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
Separate a wide range of enantiomers with Thermo Scientific™ Hypersil™ Chiral HPLC columns, designed for separations of neutral, acidic and basic racemates in normal phase. These chiral columns can help ensure your purification needs are met by resolving racemic mixtures and potential contaminants so you can be confident in the purity of your target compound. To maximize screening efficiency, these columns can be ordered in kits which contain a column in each of our chiral phases. All columns are available in analytical and preparative scale formats. Separate Enantiomers Hypersil Chiral JT • Best starting point for method development. • Chemistry: Tris-(4-methylbenzoyl)-cellulose modified silica. • USP L80. Hypersil Chiral AT • Highest generality of all chiral phases. • No aromatic ring necessary. • No inversion of elution order possible. • Chemistry: Amylose tris-3,5-dimethylphenylcarbamate modified silica. • USP L51. Hypersil Chiral OT • Highly versatile chiral phases. • No aromatic ring necessary. • No inversion of elution order possible. • Chemistry: Cellulose tris-(3.5-dimethylphenyl-carbamate) modified silica. • USP L40. Chiral Column Kits • Contain three columns, one of each Hypersil Chiral JT, Hypersil Chiral AT, and Hypersil Chiral OT. HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC.
Simultaneously separate the herbicides diquat and paraquat with Thermo ScientificTM AcclaimTM Trinity Q1 LC Columns. These tri-mode (WCX, WAX, RP) columns offer unmatched high-resolution and high-throughput trace analysis through LC-MS/MS and LC-UV methods. The stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, provides multiple retention mechanisms, including reversed-phase, anion-exchange, and cation-exchange, for maximum flexibility in method development. Easily optimize the selectivity to accelerate chromatographic separations and increase productivity. Superior Performance for Diquat and Paraquat Analysis • Distinctive spatial separation of anion-exchange and cation-exchange regions for unparalleled chromatographic performance • Adjustable selectivity using mobile phase buffer concentration, pH, and solvent content • Quick separation of hydrophilic ionic and ionizable analytes without ion-pairing reagent • Ideal selectivity for simultaneous separation of basic, neutral, and acidic analytes • Compatibility with mass spectrometry detection, which significantly improves sensitivity and provides conformation at the same time • Available in 3.0μm particle size and 2.1 and 3.0mm diameters, in 50mm length
Feature high loading capacity for exceptional suitability in the analysis of low abundant peptides in complex proteomics samples with Thermo Scientific™ PepMap™ 100 C18 LC Columns. PepMap LC columns offer lengths targeting fast or high resolution peptide separation. • High resolution in protein identification, biomarker discovery, and systems biology • Particular loadability allows highest sensitivity in LC/MS • Designed for TFA-free LC-MS, minimizing ion-suppression effects • Optimized for coupling to ESI/MS and MALDI-MS • Highest column-to-column reproducibility • Easy-to-use, cutting-edge miniaturized HPLC • Preassembled with nanoViper column inlet fittings for easy column installation Key advantages of nanoViper fittings • Ease of use, no sleeve for small capillaries • Tool-free assembly • No column damage from overtightening: capable of withstanding pressures up to 1000 bars and is compatible with third-party valves and unions • No experimental failure due to bad connections Ideal for high-resolution peptide mapping of tryptic, natural, and synthetic peptides as well as LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology. • High loading capacity • Use for the analysis of low abundant peptides often found in complex proteomics samples • Select the column length and I.D. for your throughput, sensitivity, and amount of sample available requirements • Choose from 50, 150, 250, 500mm lengths and 50, 75, 300 and 1000μm I.D. • Superior protein and peptide separations • Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity • Outstanding separation efficiency • Superior resolution and recovery • Easy installation with nanoViper™ Fingertight Fittings PepMap nanocolumns are also available pre-assembled with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fitting for a dead-volume-free connection to 1000 bar. • Requires no tools • Provides virtually zero-dead-volume connections • Withstands pressures up to a 1000 bar About Nano, capillary, and micro Acclaim PepMap columns Nano (75μm), capillary (300μm) and micro (1000μm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and microcolumns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and microcolumn in a variety of chemistries for peptides and protein analysis. Use PepMap Trap columns for desalting and preconcentration of peptides before LC separation with MS detection. The trap columns provide the highest efficiencies for one-dimensional peptide mapping experiments and 2D-LC analyses.
HYPERSIL GOLD PEI HILIC 5UM 250X10MM PREP COLUMN
Easily scale up your analytical method using Thermo Scientific™ Hypersil™ PREP BDS C18 reversed phase preparative HPLC Columns. The prep columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. These endcapped, silica-based columns offer C18 retention with base deactivation for reduced silanol activity and are an excellent choices for routine applications which require development from analytical to preparative LC scale. Excellent Choice for Routine Applications • C18 (USP L1) selectivity. • Base deactivated silica. • pH stable from 2 to 8. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
The Thermo Scientific™ MAbPac™ Protein A column is a high performance affinity chromatography column specifically designed for fast, accurate determination of monoclonal antibody concentration in the harvest cell culture. Product Highlights • A novel non-porous polymeric resin with a hydrophilic surface • Functionalized with the recombinant Protein A ligands • Wide range of MAb quantitation: 0.025 mg/mL to 5 mg/mL • Fast analysis: 2 min/analysis • Durability: up to two thousand injections per column • Low pressure: even at 2 mL/min flow rat
Use Thermo Scientific™ MAbPac™ SCX-10 LC Columns for high-resolution, high efficiency analysis of monoclonal antibodies (mAbs) and associated variants. The unique nonporous pellicular resin provides the resolving power to separate mAb variants that differ by as little as one charged residue. A hydrophilic layer surrounds the polymeric beads and eliminates hydrophobic interactions with the resin, resulting in very efficient peaks. A proprietary grafted cation-exchange surface provides pH selectivity control for high-resolution separations. Superior Performance for mAb Variant Analysis • Exceptionally high resolution for mAb variants • Ideal for characterization and quality control assessment of mAbs • Well-suited for stability studies, mAb therapeutic method development and high-throughput screening • Unmatched column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing • Meets the regulatory requirements for biopharmaceutical characterization Multiple Sizes Available • Available in 3, 5 and 10μm particle sizes in standard and UHPLC formats • Smaller-particle-size columns provide the same resolution as the 10μm columns, but with significantly faster analysis time
Maintain retention for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil™ PREP BDS Phenyl reversed phase preparative HPLC columns as you scale up your method. The stationary phase is base deactivated for reduced silanol activity and has moderate hydrophobicity, making it ideal for the separation of analyte mixtures with varying polarity and aromaticity. These preparative HPLC columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Unique Selectivity • Enhanced selectivity for aromatic and moderately polar analytes. • Base deactivated silica. • Selectivity complementary to that provided by alkyl chain phases. • Moderate hydrophobicity compared to C18. • Phenyl (USP L11) selectivity. • pH stable from 2 to 8. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. THE Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
Please fill out your contact data and your information request, we will contact you as soon as possible with all requested information.
Please fill out your contact data and your information request, we will contact you as soon as possible with all requested information.
