General purpose reverse phase separation. Specifications: • Ultra pure silica • Fully end capped optimized C18 phases
ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series. The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions. The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation. Features • High efficiency and reproducibility with short analysis time • Semi-prep and prep column dimensions • Compatible with organic modifiers and denaturants • Wide usable pH range (3-8)
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