Hypersil Chiral-HPLC-Column
Designed for the separation of hydrophilic biomolecules in HILIC mode, Thermo Scientific™ Accucore™ 150 Amide HILIC LC Columns are an excellent choice for glycan separations.Based on Core Enhanced Technology, Thermo Scientific Accucore columns provide fast, high-resolution biomolecule separations—without the elevated backpressures required by sub-2µm particles. Rugged and reproducible 2.6µm solid core particles. • Fast separations with superb resolutionLow backpressures • Amide phase bonded onto 150Å pore diameter solid core particles • High retention of a broad range of hydrophilic analytes in HILIC mode • Recommended for hydrophilic biomolecules such as glycans
Agilent AdvanceBio Amide HILIC columns are designed for HILIC separations of N-glycans and other polar analytes. The totally porous, 1.8 µm silica particles and unique bonding chemistry provide for exceptional long-term stability and reproducibility, while providing optimal separation of complex N-glycan mixtures.
Chromatography-columns, with fused-in sintered glass-disc P0, ST-PTFE-stopcock, screw-thread retaining nut, made of borosilicate glass.
Chromatography-columns, with reservoir, socket, with identations above the stopcock for a wad cotton, made of borosilicate glass 3.3.
Features and Benefits: • Pore sizes to suit your analysis (1000 Å or 120 Å). • Intermediate polarity C8 phase. • Continuity of using HPLC for all separation needs simplifies your workflow. • Facilitates the use of MS. • Eliminate SDS-PAGE from your workflow. Recommended Applications: 120 Å recommended for compounds too strongly retained on C18 phases, 1000 Å recommended for proteins. Product Specifications: 0.15, 0.3, 1, 2.1, 3, and 4.6 mm ID columns available in 50, 100, 150, 250 mm lengths. PEEK coated, and PEEKsil®, column hardware available to reduce non-specific interactions when analyzing compounds with metal chelating activity.
Features and Benefits: • Pore sizes to suit your analysis (1000 Å or 120 Å). • Intermediate polarity C8 phase. • Continuity of using HPLC for all separation needs simplifies your workflow. • Facilitates the use of MS. • Eliminate SDS-PAGE from your workflow. Recommended Applications: 120 Å recommended for compounds too strongly retained on C18 phases, 1000 Å recommended for proteins. Product Specifications: 0.15, 0.3, 1, 2.1, 3, and 4.6 mm ID columns available in 50, 100, 150, 250 mm lengths. PEEK coated, and PEEKsil®, column hardware available to reduce non-specific interactions when analyzing compounds with metal chelating activity.
General purpose reverse phase separation. Specifications: • Stable in aqueous conditions • Reduces non-specific analyte interactions • Separates polar compounds • pH stability 1 - 9
General purpose reverse phase separation. Specifications: • Modified bonded phase making it easier to work from low to high pH using the same column • Novel chemical bonding ensuring stability under extreme alkaline and acidic conditions • pH stability 1 -11
Specifikation: • Polar embedded C18 • 100 % compatible with water • pH stability 1 - 9
Features and Benefits: • Pore sizes to suit your analysis (1000 Å or 120 Å). • Intermediate polarity C8 phase. • Continuity of using HPLC for all separation needs simplifies your workflow. • Facilitates the use of MS. • Eliminate SDS-PAGE from your workflow. Recommended Applications: 120 Å recommended for compounds too strongly retained on C18 phases, 1000 Å recommended for proteins. Product Specifications: 0.15, 0.3, 1, 2.1, 3, and 4.6 mm ID columns available in 50, 100, 150, 250 mm lengths. PEEK coated, and PEEKsil®, column hardware available to reduce non-specific interactions when analyzing compounds with metal chelating activity.
Features and Benefits: • Pore sizes to suit your analysis (1000 Å or 120 Å). • Intermediate polarity C8 phase. • Continuity of using HPLC for all separation needs simplifies your workflow. • Facilitates the use of MS. • Eliminate SDS-PAGE from your workflow. Recommended Applications: 120 Å recommended for compounds too strongly retained on C18 phases, 1000 Å recommended for proteins. Product Specifications: 0.15, 0.3, 1, 2.1, 3, and 4.6 mm ID columns available in 50, 100, 150, 250 mm lengths. PEEK coated, and PEEKsil®, column hardware available to reduce non-specific interactions when analyzing compounds with metal chelating activity.
General purpose reverse phase separation. Specifications: • Stable in aqueous conditions • Reduces non-specific analyte interactions • Separates polar compounds • pH stability 1 - 9
General purpose reverse phase separation. Specifications: • Stable in aqueous conditions • Reduces non-specific analyte interactions • Separates polar compounds • pH stability 1 - 9
General purpose reverse phase separation. Specifications: • Ultra pure silica • Fully end capped optimized C18 phases
General purpose reverse phase separation. Specifications: • Ultra pure silica • Fully end capped optimized C18 phases
General purpose reverse phase separation. Specifications: • Modified bonded phase making it easier to work from low to high pH using the same column • Novel chemical bonding ensuring stability under extreme alkaline and acidic conditions • pH stability 1 -11
Features and Benefits: • Allows basic compound separation in normal phase and carbohydrate analysis. Recommended Applications: • Reverse phase carbohydrate analysis and normal phase basic compound separations.
General purpose reverse phase separation. Specifications: • Ultra pure silica • Fully end capped optimized C18 phases
ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series. The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions. The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation. Features • High efficiency and reproducibility with short analysis time • Semi-prep and prep column dimensions • Compatible with organic modifiers and denaturants • Wide usable pH range (3-8)
NOVEMA Max columns are made of NH-functionalized acrylate copolymer. These columns are suitable for the aqueous GPC/SEC analysis of cationic polymers, such as polymeric quaternary ammonium compounds, poly(DADMAC), polyvinylpyridine, chitosan, and poly(ethylene imine), with molecular weights ranging from 100 to 30,000,000 Da. Available in two particle sizes and different porosities as well as linear/mixed-bed options, NOVEMA Max columns are flexible to meet a variety of application needs. NOVEMA Max columns meet USP L23, tolerate high buffer concentrations, pH 2 to 12, temperatures up to 80 °C, and organic solvents (up to 100% methanol or acetonitrile).
EasyValid Validation Kit for organic applications. Includes calibration standards, 6 x 5 1.5 mL vials, one EasyValid column, WinGPC report layouts and import files, and comprehensive user documentation
EasyValid Validation Kit for aqueous applications. Includes calibration standards, 6 x 5 1.5 mL vials, one EasyValid column, WinGPC report layouts and import files, and comprehensive user documentation
SDV columns are useful for a wide variety of polymer analyses in nonpolar and medium polar organic solvents. These GPC columns are made from a styrene-divinylbenzene copolymer network and are available in three particle sizes (3, 5, and 10 μm) and a range of porosities to meet many organic GPC and SEC polymer analysis needs, including for polystyrene, polyvinyl chloride, polycarbonate, elastomers, resins, and more. The molecular weight range for SDV columns is 100 to 30,000,000 Da. SDV columns meet USP L21.
ZORBAX pH Method Development Kit, 4.6 x 50 mm, 1.8 µm, 600 bar; includes ZORBAX StableBond SB-C18, Eclipse Pluse C18, and Extend-C18 columns
GRAM columns can be used for organic GPC and SEC polymer analysis in polar organic solvents. These polyester copolymer-based GPC columns are available in a range of porosities, from 30 to 10,000 Å, and one linear/mixed-bed version to meet the needs for a variety of polymers with molar masses ranging from 100 to 50,000,000 Da, including for the analysis of polyurethane, polyimide, starches, cellulose, and other polar polymers.
EasyValid replacement column for aqueous applications, 8 x 300 mm. GPC/SEC column, reference materials (part number PSS-DXTVAL) and user documentation (included with kit PSS-DXTKITVAL) required for use, ships in water
POLEFIN columns are useful for high-temperature organic GPC/SEC analysis of polyolefins with molecular weights from 100 to 30,000,000 Da. These organic GPC columns are based on a modified styrene-divinylbenzene copolymer network and have a maximum temperature rating of 210 °C. POLEFIN GPC columns offer flexible configurations, including two particle sizes and two different linear/mixed-bed columns to meet the needs for a variety of polymer analyses, such as polyethylene, polypropylene, and other polyolefins.
Reversed-Phase HPLC Columns. The Agilent Pursuit family of HPLC columns offers reliable performance in various common particle sizes and phase chemistries, including USP L1, USP L7, and USP L11. Pursuit columns are available in many dimensions, including preparative HPLC columns. In addition, both traditional (Pursuit) and high carbon load (Pursuit XRS) bondings are available. Features Wide range of common particle sizes and phase chemistries, including USP L1, USP L7, and USP L11 Available in many dimensions, including preparative HPLC columns Includes both traditional (Pursuit) and high carbon load (Pursuit XRS) bondings
The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed-phase HPLC. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution.
SUPREMA columns are ideal for the aqueous GPC/SEC analysis of neutral and anionic polymers, such as PEO, PEG, pullulan, dextran, polyacrylamide, hyaluronic acid, polyacrylic acid, and carboxymethyl cellulose. These acrylate copolymer-based columns are available in three particle sizes, 3, 5, and 10 μm, individual porosities between 30 and 30,000 Å and different linear/mixed-bed columns, to meet a variety of applications needs, including polymers ranging from 100 to 30,000,000 Da. SUPREMA columns meet USP L25, L37, L38, L39, tolerate high buffer concentrations, pH 2 to 12, temperatures up to 80 °C, and organic solvents (up to 100% methanol or acetonitrile).
EasyValid replacement column for organic applications, 8 x 300 mm. GPC/SEC column, reference materials (part number PSS-PSVAL) and user documentation (included with kit PSS-PSKITVAL) required for use, ships in THF
PolarSil columns are based on polar modified silica and ideal for the organic GPC/SEC separation of low to medium molar mass resins and lignins with molecular weights from 100 to 1,000,000 Da using polar organic solvents. Multiple column configurations are available to match your specific application, including two particle sizes, 3 and 5 μm, and three porosities, 100, 300, and 1,000 Å as well as two different linear/mixed-bed columns.
Polaris - Reversed Phase HPLC Columns. Agilent Polaris HPLC columns offer many polar phase chemistries designed separate mixtures of polar and nonpolar analytes reliably and with excellent peak shapes. The polar-embedded C8 and C18 bonded phases are compatible with 100% water, and offer alternative selectivities while still complying with USP L1 and USP L7. The combination of high carbon load, ultrapure silica, and silanol shielding provides superior peak shape and retention with highly polar samples.
MCX columns are suitable for the aqueous GPC/SEC analysis of strong polyanions such as poly(styrene sulfonate), lignin sulfonate, modified starches, acids, alcohols, and pectins. This sulfonated styrene-divinylbenzene copolymer-based GPC column can be used for sulfonated polymers with molar masses ranging from 100 to 5,000,000 Da and are available in two particles sizes with a range of porosities to meet your polymer analysis needs. MCX columns meet USP L22, tolerate high buffer concentrations, pH 1 to 13, temperatures up to 80 °C, and organic solvents (up to 100% methanol or acetonitrile).
PFG columns are well suited for the organic GPC/SEC analysis of crystalline polymers with molecular weights from 100 to 3,000,000 Da using fluorinated organic solvents. These polar modified silica-based columns are available in two particle sizes, 5 and 7 μm, and four single porosities, 100, 300, 1,000, and 4,000 Å, as well as three different linear/mixed-bed columns, to meet the needs for a variety of organic GPC applications, like crystalline polymers, polyesters, polyamides, polylactides, and POM. Columns produced under DIN EN ISO 9001 quality standards Fittings and integrated diffusers provide low dead volume and efficient distribution of the sample across the entire chromatographic bed for optimal sample injection
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