Agilent AdvanceBio Peptide Mapping columns are designed to provide high-resolution peptide maps for protein identification and determination of post translation modifications. These columns provide the ability for you to quickly resolve and identify amino acid substitutions/modifications in a protein primary sequence. These advanced biocolumns are superficially porous and have a 120 Å pore size, which is optimum for the analysis of the peptides produced by enzymatic digest of proteins. The 2.7 µm particle size enables UHPLC performance on HPLC systems, which delivers high resolution separations 2–3 times faster than with fully porous HPLC columns. Each batch of the AdvanceBio Peptide Mapping media is tested with a peptide mix to ensure suitability and reproducibility, and to provide greater confidence in the analytical data generated. Use Agilent’s 10-Peptide Quality Control Standard, the same standard Agilent uses to QC its columns, to evaluate your column’s performance over its lifetime. It can be used for HPLC or LC/MS. Approximately 20 injections per vial. Order part number 5190-0583. AdvanceBio Peptide Mapping columns are the first members of Agilent’s growing state-of-the-art family of biocolumns. They are designed to deliver consistent, exceptional performance for the separation and characterization of peptides and proteins.
AdvanceBio RP-mAb columns are designed to optimize the performance of intact and reduced mAb analysis when analyzing monoclonal antibodies for biopharma discovery, development, and QA/QC applications. Analysis of intact and reduced monoclonal antibodies are critical measurements for characterizing therapeutic proteins and understanding their efficacy and stability. Poor chromatographic separations can result in rework and even compromise the accuracy of the characterization. Based on Poroshell technology with unique engineering for pore size and bonded phases, AdvanceBio RP-mAb columns deliver higher resolution and faster run times to provide accurate, reproducible results. Available in a range of chemistries – SB-C8, C4, and diphenyl. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers . Features: • Improved accuracy: Superficially porous particles (3.5 µm) with wide pores (450Å) increase mAb resolution while maintaining compatibility with all LC instruments • Speed: Shorter analysis times compared to columns packed with fully porous particles of the same size • Lower costs: The robust Poroshell packed bed and 2 µ inlet frit extend column lifetime by helping prevent inlet blockage
AdvanceBio RP-mAb columns are designed to optimize the performance of intact and reduced mAb analysis when analyzing monoclonal antibodies for biopharma discovery, development, and QA/QC applications. Analysis of intact and reduced monoclonal antibodies are critical measurements for characterizing therapeutic proteins and understanding their efficacy and stability. Poor chromatographic separations can result in rework and even compromise the accuracy of the characterization. Based on Poroshell technology with unique engineering for pore size and bonded phases, AdvanceBio RP-mAb columns deliver higher resolution and faster run times to provide accurate, reproducible results. Available in a range of chemistries – SB-C8, C4, and diphenyl. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers . Features: • Improved accuracy: Superficially porous particles (3.5 µm) with wide pores (450Å) increase mAb resolution while maintaining compatibility with all LC instruments • Speed: Shorter analysis times compared to columns packed with fully porous particles of the same size • Lower costs: The robust Poroshell packed bed and 2 µ inlet frit extend column lifetime by helping prevent inlet blockage
AdvanceBio RP-mAb columns are designed to optimize the performance of intact and reduced mAb analysis when analyzing monoclonal antibodies for biopharma discovery, development, and QA/QC applications. Analysis of intact and reduced monoclonal antibodies are critical measurements for characterizing therapeutic proteins and understanding their efficacy and stability. Poor chromatographic separations can result in rework and even compromise the accuracy of the characterization. Based on Poroshell technology with unique engineering for pore size and bonded phases, AdvanceBio RP-mAb columns deliver higher resolution and faster run times to provide accurate, reproducible results. Available in a range of chemistries – SB-C8, C4, and diphenyl. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers . Features: • Improved accuracy: Superficially porous particles (3.5 µm) with wide pores (450Å) increase mAb resolution while maintaining compatibility with all LC instruments • Speed: Shorter analysis times compared to columns packed with fully porous particles of the same size • Lower costs: The robust Poroshell packed bed and 2 µ inlet frit extend column lifetime by helping prevent inlet blockage
HC-C18, 4.6 x 250 mm, 5 u, 2.5 ml solvent, 4.2 ml tube
Eclipse XDB Columns • provide excellent separations over a wide pH range (pH 2 to pH 9), • provide reliable performance under high pressure and flow - a result of Agilent's proprietary ZORBAX silica gel particles, • enable flexible method development with many choices in four bonded phases and a wide range of particle sizes and dimensions.
Eclipse XDB Columns • provide excellent separations over a wide pH range (pH 2 to pH 9), • provide reliable performance under high pressure and flow - a result of Agilent's proprietary ZORBAX silica gel particles, • enable flexible method development with many choices in four bonded phases and a wide range of particle sizes and dimensions.
The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed-phase HPLC. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution. Features • Traditional, fully porous particles available in 1.8, 3.5, and 5 µm sizes. With selected products also available in 7 μm particles for preparative HPLC • ZORBAX Extend-C18 columns offer high efficiency for high pH applications up to pH 11.5
ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series. The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions. The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation. Features • High efficiency and reproducibility with short analysis time • Semi-prep and prep column dimensions • Compatible with organic modifiers and denaturants • Wide usable pH range (3-8)
ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series. The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions. The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation. Features • High efficiency and reproducibility with short analysis time • Semi-prep and prep column dimensions • Compatible with organic modifiers and denaturants • Wide usable pH range (3-8)
Low-cost C18 columns for legacy HPLC applications. Available in moderate and high carbon loads.
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Features • Agilent's recommended column for carbohydrate analysis • Operates on a standard HPLC and does not require specialized equipment • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier • Available in 8 µm and 10 µm particle sizes • Complies with USP L17, USP L19, and USP L34
Hypersil AA-ODS, 5 µm, 2.1 x 200 mm. 400 bar pressure limit
Hypersil ODS (5021-1845 cartridge holder required (AC))
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