Scale up your analytical method using Thermo Scientific™ Hypersil™ PREP ODS C18 reversed phase preparative HPLC Columns. These columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. This C18 column is a general purpose column used in a broad range of applications, perfect for routine workflows. For Routine Applications • C18 (USP L1) selectivity. • pH stable from 2 to 8. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
The Thermo Scientific™ NativePac OBE-1 Column is a Size Exclusion Chromatography (SEC) column designed for rapid exchange of non-volatile buffer components with a volatile buffer for Native-MS analysis of biomacromolecules. The small pores of the SEC stationary phase maximize separation of the non-volatile components from the sample of interest while the short column length minimizes analysis times to less than 2 minutes. A narrow column ID enables the use of low flow rates for high sensitivity detection and maximizes up time by minimizing buffer consumption.
ACCLAIM TRINITY XL P1 150X25 MM PREP COLUMN
Acclaim™ Phenyl-1 Reversed Phase LC Columns offer high selectivity of aromatic compounds. The specially developed bonded phase shows a higher π-π interaction than other phenyl phases and offers improved aromatic selectivity and higher hydrophobic retention. These columns are suitable is ideal for the analysis of aromatic analytes such as glucocorticosteroids, estrogens, fat-soluble vitamins and phospholipids. High aromatic selectivity for the separation of aromatic compounds • High hydrophobic retention • Compatibility with highly aqueous mobile phases • High efficiency and robust packaging • Extremely low column bleed, fully compatible with MS • Stable in the pH range from 2 to 8 • Available in particle sizes of 3 and 5.0 μm, with diameters of 4.6, 3.0 and 2.1 mm and an average pore diameter of 120 Å
Achieve baseline separation of carbamate pesticides (N-methylcarbamate and N-methylcarbamoyloxime pesticides) specified in U.S. EPA Method 531.2 by using Thermo Scientific™ Acclaim™ Carbamate LC Columns. These high-efficiency columns are compatible with both binary (methanol/water) and ternary (acetonitrile/methanol/water) mobile phase gradients for greater flexibility. Use these extremely low bleed columns with either LC/post-column derivatization/fluorescence or LC/MS detection for the ultimate sensitivity. Multiple Column Formats Meet Your Specific Analysis Needs • 5μm, 4.6 × 250mm Column: Sets the standard for the highest resolution with post-column reaction (PCR) and fluorescence detection (FLD) • 3μm, 4.6×150mm Column: Saves time and increases sample throughput • 3μm, 3.0×150mm Column: Saves time and reduces solvent consumption • 3μm, 2.1×150mm Column: Suited for LC/MS, to achieve the lowest possible detection limits • Two RSLC, 2.2μm Columns (2.1×100mm and 2.1×150mm): Provide high-resolution, high-throughput separations
Ensure robust nano and capillary flow into your LC-MS using Thermo Scientific™ EASY-Spray™ HPLC Columns. The integrated column/emitter design eliminates dead volume and is temperature-controlled for maximum reliability and performance. Rigorously tested to ensure maximum quality, these columns deliver maximum simplicity and ease-of-use. The capillary flow HPLC columns provide sensitive protein, peptide, and monoclonal antibody (mAb) separation. They give proteomics researchers more than ever before: more throughput, more sensitivity, more separation power and more ease of use. The unique EASY-Spray design provides uncompromised performance and ease-of-use in nano and capillary LC-MS proteomics applications. Features include the following: • Precision positioned glass emitter • Integrated precision designed union • Integrated temperature control
Ensure robust nano and capillary flow into your LC-MS using Thermo Scientific™ EASY-Spray™ HPLC Columns. The integrated column/emitter design eliminates dead volume and is temperature-controlled for maximum reliability and performance. Rigorously tested to ensure maximum quality, these columns deliver maximum simplicity and ease-of-use. The capillary flow HPLC columns provide sensitive protein, peptide, and monoclonal antibody (mAb) separation. They give proteomics researchers more than ever before: more throughput, more sensitivity, more separation power and more ease of use. The unique EASY-Spray design provides uncompromised performance and ease-of-use in nano and capillary LC-MS proteomics applications. Features include the following: • Precision positioned glass emitter • Integrated precision designed union • Integrated temperature control
Thermo Scientific™ Acclaim™ HILIC-10 LC Columns separate highly hydrophilic molecules using hydrophilic interaction liquid chromatography. These columns enable the retention and separation of highly polar molecules that are not retained by reversed-phase chromatography. The high-purity, Spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry allows the use of 5 to 20% of an aqueous mobile phase while maintaining affinity for polar analytes. Sample throughput is increased without the need for non-aqueous To evaporate or reconstitute samples dissolved in solvents. Robust and reproducible separations of strongly polar compounds: • Unique selectivity, complementing reversed phase columns • Improved sensitivity in MS detection • Hydrolytically stable • Robust column packaging • Wide range of applications including separation of hydrophilic drugs and their metabolites Acclaim HILIC-10 LC columns are available in a 3 μm particle size and a variety of column formats: 4.6 × 150 mm, for routine, high-throughput analysis; 3.0 × 150 mm, for high throughput analysis and reduced solvent consumption; 2.1 × 150 mm, for LC-MS analysis.
For separations of hydrophobic, structurally related isomers, Thermo Scientific™ Acclaim™ C30 LC reversed-phase columns provide superior results. The covalent modification of C30 alkyl silane on ultrapure, spherical, porous silica gel gives these columns high shape selectivity. They are fully compatible with various aqueous buffers, useful for a broad application range of analytes, and offer more flexibility in method development. High Shape Selectivity for the Separation of Structurally Related Isomers • Unique selectivity complementary to other reversed-phase columns • High efficiency and rugged packing • Extremely low bleed, fully compatible with MS • Stable between pH 2 and 8 • Available in 3 and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 200Å
Maintain exceptional peak shape and resolution for your scale up purification needs using Thermo Scientific™ Hypersil GOLD™ C18 preparative HPLC columns. Our columns come in a variety of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. Hypersil GOLD columns give you confidence when scaling up from your analytical method. Novel Bonding • Proprietary functionalization and end-capping. • C18 (USP L1) selectivity. pH Stability • Robust performance at high pH. • Excellent column stability at low pH. Superior Performance • Highly symmetrical peak shapes. • Improved speed of analysis, efficiency, and sensitivity. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder. Customer Specific Orders • If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in a preparative scale not shown or would like to discuss a specific column dimension, we would be happy to discuss your requirements. • Please contact your local sales representative for Customer Specific Orders. • Custom built columns are not eligible for returns.
Separate challenging peptide mapping samples with Thermo Scientific™ Double nanoViper™ PepMap™ Neo UHPLC columns. These columns feature easy connectivity, high reproducibilty, and excellent separations. Our Neo columns are packed to higher pressure and provide 1500 bar pressure capability, improved column-to-column consistency, and increased efficiency. The column media is manufactured and selected to exacting standards and packed at high pressure, resulting in enhanced peak symmetry, resolution, and column-to-column reproducibility that allows you to obtain greater sample coverage and sample insights. Benefits of PepMap Neo Columns • Brilliant Connectivity — Double nanoViper™ columns connect with ease. • Brilliant Reproducibility — Precise manufacturing protocols for packing media improve column-to-column consistency for reproducible analysis every day. • Brilliant Separation — Ultra-high pressure packing increases column efficiency, allowing you to run at up to 1500 bar for enhanced peak symmetry and resolution, providing higher sample capacity for more sample insights.
Thermo Scientific ™ Acclaim™ HAA is a silica-based application-specific column for high resolution and high throughput trace analysis of haloacetic acids (HAAs) in water samples by LC-MS. 2. Main features • Excellent resolution • Fast analysis • Ideal selectivity • LC-MS compatibility 3. Physical data • Column Chemistry: Proprietary mixed-mode • Silica Substrate: Spherical, high-purity, porous
Use Thermo Scientific™ Acclaim™ Carbonyl C18 LC Columns for the separation of dinitrophenylhydrazine (DNPH) derivatives of aldehydes and ketones with excellent resolution and high throughput. This silica-based reversed-phase column provides excellent selectivity and baseline resolution of DNPH derivatives , which are regulated by various official methods, including US EPA 554, EPA 8315, EPA 1667, EPA TO-11 and CARB 1004. Outstanding performance in demanding aldehyde and ketone analyses • Highly efficient separations enable excellent UHPLC performance • Robust columns with good reproducibility over multiple batches • Proven robust methods A variety of column dimensions to meet your analysis needs • Particle sizes: 2.2, 3 and 5 µm; Smaller particle sizes ensure high peak efficiency for quick analysis • Diameter: 2.1, 3.0 and 4.6 mm; Small diameter columns (2.1 and 3.0 mm) use less solvent and produce less chemical waste • Lengths: 100, 150 and 250 mm For high throughput applications, use the 2.1 × 100 mm column format combined with high linear speed. If high resolution performance is required, use a longer column (2.1 × 150 mm) at an optimal linear speed to achieve even better resolution.
Easily scale up your analytical method using Thermo Scientific™ Hypersil™ PREP BDS Si preparative HPLC columns which are packed with a base deactivated silica for normal phase chromatography of nonpolar and moderately polar organic compounds.These preparative HPLC columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Normal phase chromatography • Base deactivated silica to separate nonpolar and moderately polar organic compounds. • USP L3. • pH stable from 2 to 7. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
Easily scale up your analytical method and achieve maximum retention and loading using Thermo Scientific™ Hypersil™ PREP HS Si preparative HPLC columns. Packed with a bare silica, the high surface area particles with a 100Å pore size are made for normal phase chromatography of nonpolar and moderately polar organic compounds. These preparative HPLC columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Normal phase chromatography • Silica to separate nonpolar and moderately polar organic compounds. • USP L3 - high surface area particle with 100Å pore size. • pH stable from 2 to .7 • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. These preparative guard columns require the use of a preparative guard holder.
Get a high-performance, reversed-phase columns for aminoglycoside analysis. The robust and rugged Thermo Scientific™ Acclaim™ AmG C18 HPLC column provides exceptional tolerance required for aminoglycosides ion-pairing chromatographic separations. These silica-based C18 columns are optimized for various aminoglycoside antibiotic analyses, including drug purity characterization and quantification, therapeutic drug monitoring, and residual control testing in different matrices. These columns are stable at low pH, high temperature, organic solvents, and aqueous mobile phases. Highly Stable for Aminoglycoside Analyses The Acclaim AmG C18 column is specifically designed for ion-pairing reversed-phase HPLC (IP-RPLC) analysis of aminoglycoside antibiotics using volatile perfluorinated carboxylic acids as the ion-pairing reagent, such as 100 mM trifluoroacetic acid (TFA). The Acclaim AmG C18 stationary phase is based on a proprietary C18 bonding technology that provides extremely high stability towards acidic conditions. This specialty column exhibits excellent selectivity and high resolution for the aminoglycoside antibiotics analysis. In addition, it is easy to use because simple aqueous mobile phase (e.g. 100 mM TFA) without organic solvent and pH adjusting buffer is sufficient in most cases. Excellent selectivity for aminoglycosides Superior tolerance towards acidic conditions High efficiency and throughput Ease of use The column can be used with UV, CAD, ELSD detectors
HYPERSIL GOLD SILICA 5UM 250X10MMPREP COLUMN
HYPERSIL GOLD SI 5UM 250X20MMPREP COLUMN
Maintain the sensitivity you need for your purification needs using Thermo Scientific™ Hypersil GOLD™ C8 preparative LC Columns. Best for scale up from analytical to prep LC method and ideal for analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention. The lower hydrophobicity allows compounds to elute more quickly, yielding faster separations. Benefit from similar selectivity to C18 columns with a predictable elution order. Our columns come in a variety of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Similar selectivity, less retention than C18 columns • Successful retention and separation of moderately hydrophobic analytes. • Best for fatty acids, which are too hydrophobic for analysis using C18 columns. • C8 (USP L7) selectivity. Faster Separations • Higher analytical throughput compared to C18 alkyl chain chemistry. • Faster compound elution due to reduced hydrophobic interactions. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.
HYPERSIL GOLD PFP 5UM 150 X 4MM CC
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