Hypersil PREP HS phenyl chemistry with high loading capacity and fully scalable from analytical to prep, suitable for all USP-L11 applications. A high surface area particle with a 100Å pore size and a phenyl bonded phase gives the stationary phase moderate hydrophobicity, making it ideal for the separation of analyte mixtures with varying polarity and aromaticity perfect for small molecule and drug purification needs. Unique Selectivity • Enhanced selectivity for aromatic and moderately polar analytes. • Selectivity complementary to that provided by alkyl chain phases. • Moderate hydrophobicity compared to C18. • Phenyl (USP L11) selectivity - high surface area particle with 100Å pore size. • pH stable from 2 to 8. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. These preparative guard columns require the use of a preparative guard holder.
Thermo Scientific™ Acclaim™ Vanquish™ C18 UHPLC Columns ensure improved separation performance and speed as well as higher throughput. These reversed phase columns were developed in conjunction with the Vanquish UHPLC system to take advantage of the system's expanded printing capabilities and robustness. The result is a new level of separation, speed and throughput that solves analytical challenges in liquid chromatography (LC) and LC-MS analyses. For powerful separations The expanded offering of Thermo Scientific™ Accucore™ Vanquish™ and Acclaim VANQUISH UHPLC columns provides a greater range of selectivity and format options to meet the diverse needs of UHPLC applications. They offer: • Better separations: high efficiency enables the separation of very complex mixtures • Further results: High efficiency at high flow rates • Easier Interaction: Seamless workflow solution for easy and hassle-free disconnects • Advanced features: Wide range of selectivities and formats
Achieve even greater power of separation, speed, and throughput of polar and nonpolar compounds in a single run using Thermo Scientific™ Acclaim™ VANQUISH™ PA2 UHPLC columns. These reversed-phase columns were developed in conjunction with the Vanquish UHPLC system to take advantage of the system's extended pressure capabilities and robustness. The result is a new level of separation, speed and throughput that solve analytical challenges in liquid chromatography (LC) and LC-MS analyses. These Polar Advantage II columns provide selectivity that is complementary to conventional C18 columns. Delivering Powerful Separations The expanded range of Thermo Scientific™ Accucore™ Vanquish™ and Acclaim VANQUISH UHPLC columns offer a wider range of selectivity and format options to satisfy different UHPLC application requirements. They deliver: Better Separations: high efficiency enables separation of very complex mixture More Results: High efficiency at high flow rates Easier Interactions: Seamless workflow solution, for simple and easy separations Wider Capabilities: Wide range of column selectivities and formats
Separate water-soluble polymers and oligomers with Thermo Scientific™ Acclaim™ SEC-1000 Size Exclusion HPLC Columns. These resin-based, high-performance, size-exclusion chromatography columns cover molecular weight range: 7μm, 1000Å. The combination of a proprietary mono-dispersed multi-pore hydrophilic resin and small particle size provides high-resolution and good linearity of the calibration curve with no inflection points for accurate, reliable analysis of a variety of water-soluble polymers. The Acclaim SEC-1000 LC column is calibrated from 1,000 to 1,000,000 Daltons and can characterize high molecular weight polymers. High-Performance Analysis of Water-Soluble Polymers High-resolution analysis at reduced solvent consumption in available 4.6 x 300mm dimension Stable surface bonding leads to low column bleed and enhances compatibility with UV, RI, MS, ELSD and Thermo Scientific Dionex™ Corona™ Charged Aerosol Detectors
Preperative Hypersil GOLD aQ polar endcapped C18 columns are stable in 100% aqueous mobile phase and provide enhanced retention and resolution of polar analytes. The HPLC columns come in a wide range of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Alternative selectivity with polar endcapped C18 stationary phase • Polar functional group in endcap provides further interaction mechanism for separation of polar compounds. • Yields superior retention of polar compounds. • C18 (USP L1) selectivity. Stability in highly aqueous mobile phases • Applicable in 100% aqueous mobile phases. • No loss of performance or stability. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.
Separate water-soluble polymers and oligomers with Thermo Scientific™ Acclaim™ SEC-300 Size Exclusion Chromatography HPLC Columns. These resin-based, high-performance, size-exclusion chromatography columns cover molecular weight range: 5μm, 300Å. The combination of a proprietary mono-dispersed multi-pore hydrophilic resin and small particle size provides high-resolution and good linearity of the calibration curve with no inflection points for accurate, reliable analysis of a variety of water-soluble polymers. The Acclaim SEC-300 column is calibrated from 100 to 50,000 Daltons and offers good resolution for lower molecular weight polymers or oligomers. High-Performance Analysis of Water-Soluble Polymers • High-resolution analysis at reduced solvent consumption in available 4.6 x 300mm dimension • Stable surface bonding leads to low column bleed and enhances compatibility with UV, RI, MS, ELSD and Thermo Scientific Dionex™ Corona™ Charged Aerosol Detectors
Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific™ Acclaim™ Trinity P2 LC Columns. The innovative, mixed-mode column technology provides hydrophilic interaction, HILIC, anion-exchange, and cation-exchange functionalities for flexible method development. Use the Acclaim Trinity P2 LC column to complement the Acclaim Trinity P1 LC column, which simultaneously separates pharmaceutical drug substances and their counterions, to provide a total solution for pharmaceutical counterion analysis by HPLC. The Acclaim Trinity P2 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical silica particles, coated with charged nanopolymer particles. The unique surface chemistry includes an inner-pore area of the silica bead modified with a covalently bonded organic layer that provides cation-exchange retention. The outer-pore surface is modified with anion-exchange nano-polymer beads. This chemistry ensures spatial separation of the anion-exchange and cation-exchange regions. High Selectivity for Counterion Analysis • Low column bleed; compatible with mass spectrometry (MS) and charged aerosol detection (CAD) • Hydrolytically stable • High efficiency • Available in 3.0μm particle size and 2.1 and 3.0mm diameters, in 50 and 100mm lengths
HYPERSIL GOLD PFP 5UM PREP COLUMN
Attain faster separations for hydrophobic analytes when you scale up using Thermo Scientific™ Hypersil GOLD™ C4 reversed phase preparative HPLC Columns, for your purification needs. These C4 columns provide excellent sensitivity and high efficiency, highlighting a lower hydrophobicity than C8 or C18 columns. These columns come in a variety of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Similar selectivity, less retention than C18 or C8 columns • Outstanding retention and separation of very hydrophobic analytes with this short alkyl chain length, low hydrophobicity column. • Best for analysis of hydrophobic polypeptides and proteins. • C4 (USP L26) selectivity. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d.
HYPERSIL GOLD CN 5UM PREP Column
HYPERSIL GOLD SAX 5UM 150X20MM PREP Column
Use Thermo Scientific™ Hypersil™ PREP Si preparative HPLC columns to scale up your normal phase chromatography analytical method. The silica chemistry separates nonpolar and moderately polar organic compounds. These preparative HPLC columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Normal phase chromatography Silica to separate nonpolar and moderately polar organic compounds. USP L3. pH stable from 2 to 7. Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. Standard threaded hardware for column i.d. < 40mm. Standard flange hardware for column i.d. ≥ 40mm. Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
Separate a wide range of enantiomers with Thermo Scientific™ Hypersil™ Chiral HPLC columns, designed for separations of neutral, acidic and basic racemates in normal phase. These chiral columns can help ensure your purification needs are met by resolving racemic mixtures and potential contaminants so you can be confident in the purity of your target compound. To maximize screening efficiency, these columns can be ordered in kits which contain a column in each of our chiral phases. All columns are available in analytical and preparative scale formats. Separate Enantiomers Hypersil Chiral JT • Best starting point for method development. • Chemistry: Tris-(4-methylbenzoyl)-cellulose modified silica. • USP L80. Hypersil Chiral AT • Highest generality of all chiral phases. • No aromatic ring necessary. • No inversion of elution order possible. • Chemistry: Amylose tris-3,5-dimethylphenylcarbamate modified silica. • USP L51. Hypersil Chiral OT • Highly versatile chiral phases. • No aromatic ring necessary. • No inversion of elution order possible. • Chemistry: Cellulose tris-(3.5-dimethylphenyl-carbamate) modified silica. • USP L40. Chiral Column Kits • Contain three columns, one of each Hypersil Chiral JT, Hypersil Chiral AT, and Hypersil Chiral OT. HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC.
Simultaneously separate the herbicides diquat and paraquat with Thermo ScientificTM AcclaimTM Trinity Q1 LC Columns. These tri-mode (WCX, WAX, RP) columns offer unmatched high-resolution and high-throughput trace analysis through LC-MS/MS and LC-UV methods. The stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, provides multiple retention mechanisms, including reversed-phase, anion-exchange, and cation-exchange, for maximum flexibility in method development. Easily optimize the selectivity to accelerate chromatographic separations and increase productivity. Superior Performance for Diquat and Paraquat Analysis • Distinctive spatial separation of anion-exchange and cation-exchange regions for unparalleled chromatographic performance • Adjustable selectivity using mobile phase buffer concentration, pH, and solvent content • Quick separation of hydrophilic ionic and ionizable analytes without ion-pairing reagent • Ideal selectivity for simultaneous separation of basic, neutral, and acidic analytes • Compatibility with mass spectrometry detection, which significantly improves sensitivity and provides conformation at the same time • Available in 3.0μm particle size and 2.1 and 3.0mm diameters, in 50mm length
Feature high loading capacity for exceptional suitability in the analysis of low abundant peptides in complex proteomics samples with Thermo Scientific™ PepMap™ 100 C18 LC Columns. PepMap LC columns offer lengths targeting fast or high resolution peptide separation. • High resolution in protein identification, biomarker discovery, and systems biology • Particular loadability allows highest sensitivity in LC/MS • Designed for TFA-free LC-MS, minimizing ion-suppression effects • Optimized for coupling to ESI/MS and MALDI-MS • Highest column-to-column reproducibility • Easy-to-use, cutting-edge miniaturized HPLC • Preassembled with nanoViper column inlet fittings for easy column installation Key advantages of nanoViper fittings • Ease of use, no sleeve for small capillaries • Tool-free assembly • No column damage from overtightening: capable of withstanding pressures up to 1000 bars and is compatible with third-party valves and unions • No experimental failure due to bad connections Ideal for high-resolution peptide mapping of tryptic, natural, and synthetic peptides as well as LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology. • High loading capacity • Use for the analysis of low abundant peptides often found in complex proteomics samples • Select the column length and I.D. for your throughput, sensitivity, and amount of sample available requirements • Choose from 50, 150, 250, 500mm lengths and 50, 75, 300 and 1000μm I.D. • Superior protein and peptide separations • Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity • Outstanding separation efficiency • Superior resolution and recovery • Easy installation with nanoViper™ Fingertight Fittings PepMap nanocolumns are also available pre-assembled with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fitting for a dead-volume-free connection to 1000 bar. • Requires no tools • Provides virtually zero-dead-volume connections • Withstands pressures up to a 1000 bar About Nano, capillary, and micro Acclaim PepMap columns Nano (75μm), capillary (300μm) and micro (1000μm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and microcolumns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and microcolumn in a variety of chemistries for peptides and protein analysis. Use PepMap Trap columns for desalting and preconcentration of peptides before LC separation with MS detection. The trap columns provide the highest efficiencies for one-dimensional peptide mapping experiments and 2D-LC analyses.
HYPERSIL GOLD PEI HILIC 5UM 250X10MM PREP COLUMN
Easily scale up your analytical method using Thermo Scientific™ Hypersil™ PREP BDS C18 reversed phase preparative HPLC Columns. The prep columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. These endcapped, silica-based columns offer C18 retention with base deactivation for reduced silanol activity and are an excellent choices for routine applications which require development from analytical to preparative LC scale. Excellent Choice for Routine Applications • C18 (USP L1) selectivity. • Base deactivated silica. • pH stable from 2 to 8. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
The Thermo Scientific™ MAbPac™ Protein A column is a high performance affinity chromatography column specifically designed for fast, accurate determination of monoclonal antibody concentration in the harvest cell culture. Product Highlights • A novel non-porous polymeric resin with a hydrophilic surface • Functionalized with the recombinant Protein A ligands • Wide range of MAb quantitation: 0.025 mg/mL to 5 mg/mL • Fast analysis: 2 min/analysis • Durability: up to two thousand injections per column • Low pressure: even at 2 mL/min flow rat
Use Thermo Scientific™ MAbPac™ SCX-10 LC Columns for high-resolution, high efficiency analysis of monoclonal antibodies (mAbs) and associated variants. The unique nonporous pellicular resin provides the resolving power to separate mAb variants that differ by as little as one charged residue. A hydrophilic layer surrounds the polymeric beads and eliminates hydrophobic interactions with the resin, resulting in very efficient peaks. A proprietary grafted cation-exchange surface provides pH selectivity control for high-resolution separations. Superior Performance for mAb Variant Analysis • Exceptionally high resolution for mAb variants • Ideal for characterization and quality control assessment of mAbs • Well-suited for stability studies, mAb therapeutic method development and high-throughput screening • Unmatched column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing • Meets the regulatory requirements for biopharmaceutical characterization Multiple Sizes Available • Available in 3, 5 and 10μm particle sizes in standard and UHPLC formats • Smaller-particle-size columns provide the same resolution as the 10μm columns, but with significantly faster analysis time
Maintain retention for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil™ PREP BDS Phenyl reversed phase preparative HPLC columns as you scale up your method. The stationary phase is base deactivated for reduced silanol activity and has moderate hydrophobicity, making it ideal for the separation of analyte mixtures with varying polarity and aromaticity. These preparative HPLC columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Unique Selectivity • Enhanced selectivity for aromatic and moderately polar analytes. • Base deactivated silica. • Selectivity complementary to that provided by alkyl chain phases. • Moderate hydrophobicity compared to C18. • Phenyl (USP L11) selectivity. • pH stable from 2 to 8. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. THE Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
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