Achieve superior reversed phase oligonucleotide separations using the Thermo Scientific™ DNAPac™ RP HPLC columns. The unique chemistry is designed for analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC-UV or LC-MS. The column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions. In addition, the wide pore size of the resin provides excellent separation of large double-stranded nucleic acids up to 10k base pairs. High Performance Reversed-Phase LC Columns The DNAPac RP column is based on spherical wide-pore 4μm polymer resin. The phase is highly stable to extremes of pH (0-14), and temperature (5-110°C), and operates with mobile phases compatible with direct mass spectrometry. The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments. DNAPac RP reversed phase (RP) column is specifically designed for separation of oligonucleotides and DNA/RNA fragments. The stationary phase is fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of large nucleic acids with low carry-over. Columns designed for reversed phase oligonucleotide LC and LC/MS separations • Designed for ion-pair reversed-phase (IP-RP) separations of both oligonucleotides and double-stranded nucleic acids • High resolution and high throughput • Excellent MS compatibility • Wide operating pH range (0-14) and high temperature stability (up to 110°C)
Simultaneously separate pharmaceutical drug substances and their counterions with high-efficiency Thermo Scientific™ Acclaim™ Trinity P1 LC Columns. A unique trimodal surface chemistry provides concurrent reversed-phase, cation-exchange and anion-exchange functionalities for maximum flexibility in method development. Easily optimize selectivity by adjusting mobile phase buffer concentration, pH and solvent content—either jointly or independently—to accelerate chromatographic separations and increase productivity. The Acclaim Trinity P1 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical 3μm silica particles, coated with charged nanopolymer beads. The unique surface chemistry includes an inner-pore area modified with an organic layer that provides both reversed-phase and anion-exchange properties. The outer-pore surface, conversely, is modified with cation-exchange functionality for unparalleled chromatographic performance. Superior Performance for Pharmaceutical Applications Ideal selectivity for simultaneous separation of Active Pharmaceutical Ingredients (API) and counterions (basic, neutral and acidic analytes) Effective retention of hydrophilic ionic and ionizable analytes without ion-pairing reagents Distinctive spatial separation of the anion-exchange and cation-exchange regions Flexible method development with each retention mechanism controlled independently Low column bleed; compatible with mass spectrometry (MS), charged aerosol detection (CAD) and evaporative light scattering detectors (ELSD) Available in 2.1 and 3.0mm diameters and 50, 100 and 150mm lengths Complement the Acclaim Trinity P1 column with the Acclaim Trinity P2 column to provide a total solution for pharmaceutical counterion analysis by HPLC. Use the Acclaim Trinity P2 column to separate counterions, including monovalent and divalent cations or anions.
Acclaim™ Phenyl-1 Reversed Phase LC Columns offer high selectivity of aromatic compounds. The specially developed bonded phase shows a higher π-π interaction than other phenyl phases and offers improved aromatic selectivity and higher hydrophobic retention. These columns are suitable is ideal for the analysis of aromatic analytes such as glucocorticosteroids, estrogens, fat-soluble vitamins and phospholipids. High aromatic selectivity for the separation of aromatic compounds • High hydrophobic retention • Compatibility with highly aqueous mobile phases • High efficiency and robust packaging • Extremely low column bleed, fully compatible with MS • Stable in the pH range from 2 to 8 • Available in particle sizes of 3 and 5.0 μm, with diameters of 4.6, 3.0 and 2.1 mm and an average pore diameter of 120 Å
Javelin guard columns offer convenient, easy to use protection for analytical HPLC columns Description • Direct-connection, patented fingertight design has a 1/16 in. CPI tip that attaches directly to the inlet of the HPLC column with tubing or wrenches • Requires no holder, allowing quick and easy replacement Hypersil GOLD aQ columns provide enhanced retention and resolution of polar analytes. • Polar endcapped C18 phase for alternative selectivity • Retention and resolution of polar analytes • Ideal for highly aqueous mobile phase • Excellent peak shape
Thermo Scientific™ Acclaim™ HILIC-10 LC Columns separate highly hydrophilic molecules using hydrophilic interaction liquid chromatography. These columns enable the retention and separation of highly polar molecules that are not retained by reversed-phase chromatography. The high-purity, Spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry allows the use of 5 to 20% of an aqueous mobile phase while maintaining affinity for polar analytes. Sample throughput is increased without the need for non-aqueous To evaporate or reconstitute samples dissolved in solvents. Robust and reproducible separations of strongly polar compounds: • Unique selectivity, complementing reversed phase columns • Improved sensitivity in MS detection • Hydrolytically stable • Robust column packaging • Wide range of applications including separation of hydrophilic drugs and their metabolites Acclaim HILIC-10 LC columns are available in a 3 μm particle size and a variety of column formats: 4.6 × 150 mm, for routine, high-throughput analysis; 3.0 × 150 mm, for high throughput analysis and reduced solvent consumption; 2.1 × 150 mm, for LC-MS analysis.
Thermo Scientific ™ Acclaim™ HAA is a silica-based application-specific column for high resolution and high throughput trace analysis of haloacetic acids (HAAs) in water samples by LC-MS. 2. Main features • Excellent resolution • Fast analysis • Ideal selectivity • LC-MS compatibility 3. Physical data • Column Chemistry: Proprietary mixed-mode • Silica Substrate: Spherical, high-purity, porous
For separations of hydrophobic, structurally related isomers, Thermo Scientific™ Acclaim™ C30 LC reversed-phase columns provide superior results. The covalent modification of C30 alkyl silane on ultrapure, spherical, porous silica gel gives these columns high shape selectivity. They are fully compatible with various aqueous buffers, useful for a broad application range of analytes, and offer more flexibility in method development. High Shape Selectivity for the Separation of Structurally Related Isomers • Unique selectivity complementary to other reversed-phase columns • High efficiency and rugged packing • Extremely low bleed, fully compatible with MS • Stable between pH 2 and 8 • Available in 3 and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 200Å
Use Thermo Scientific™ Acclaim™ Carbonyl C18 LC Columns for the separation of dinitrophenylhydrazine (DNPH) derivatives of aldehydes and ketones with excellent resolution and high throughput. This silica-based reversed-phase column provides excellent selectivity and baseline resolution of DNPH derivatives , which are regulated by various official methods, including US EPA 554, EPA 8315, EPA 1667, EPA TO-11 and CARB 1004. Outstanding performance in demanding aldehyde and ketone analyses • Highly efficient separations enable excellent UHPLC performance • Robust columns with good reproducibility over multiple batches • Proven robust methods A variety of column dimensions to meet your analysis needs • Particle sizes: 2.2, 3 and 5 µm; Smaller particle sizes ensure high peak efficiency for quick analysis • Diameter: 2.1, 3.0 and 4.6 mm; Small diameter columns (2.1 and 3.0 mm) use less solvent and produce less chemical waste • Lengths: 100, 150 and 250 mm For high throughput applications, use the 2.1 × 100 mm column format combined with high linear speed. If high resolution performance is required, use a longer column (2.1 × 150 mm) at an optimal linear speed to achieve even better resolution.
Get a high-performance, reversed-phase columns for aminoglycoside analysis. The robust and rugged Thermo Scientific™ Acclaim™ AmG C18 HPLC column provides exceptional tolerance required for aminoglycosides ion-pairing chromatographic separations. These silica-based C18 columns are optimized for various aminoglycoside antibiotic analyses, including drug purity characterization and quantification, therapeutic drug monitoring, and residual control testing in different matrices. These columns are stable at low pH, high temperature, organic solvents, and aqueous mobile phases. Highly Stable for Aminoglycoside Analyses The Acclaim AmG C18 column is specifically designed for ion-pairing reversed-phase HPLC (IP-RPLC) analysis of aminoglycoside antibiotics using volatile perfluorinated carboxylic acids as the ion-pairing reagent, such as 100 mM trifluoroacetic acid (TFA). The Acclaim AmG C18 stationary phase is based on a proprietary C18 bonding technology that provides extremely high stability towards acidic conditions. This specialty column exhibits excellent selectivity and high resolution for the aminoglycoside antibiotics analysis. In addition, it is easy to use because simple aqueous mobile phase (e.g. 100 mM TFA) without organic solvent and pH adjusting buffer is sufficient in most cases. Excellent selectivity for aminoglycosides Superior tolerance towards acidic conditions High efficiency and throughput Ease of use The column can be used with UV, CAD, ELSD detectors
Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific™ Acclaim™ Trinity P2 LC Columns. The innovative, mixed-mode column technology provides hydrophilic interaction, HILIC, anion-exchange, and cation-exchange functionalities for flexible method development. Use the Acclaim Trinity P2 LC column to complement the Acclaim Trinity P1 LC column, which simultaneously separates pharmaceutical drug substances and their counterions, to provide a total solution for pharmaceutical counterion analysis by HPLC. The Acclaim Trinity P2 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical silica particles, coated with charged nanopolymer particles. The unique surface chemistry includes an inner-pore area of the silica bead modified with a covalently bonded organic layer that provides cation-exchange retention. The outer-pore surface is modified with anion-exchange nano-polymer beads. This chemistry ensures spatial separation of the anion-exchange and cation-exchange regions. High Selectivity for Counterion Analysis • Low column bleed; compatible with mass spectrometry (MS) and charged aerosol detection (CAD) • Hydrolytically stable • High efficiency • Available in 3.0μm particle size and 2.1 and 3.0mm diameters, in 50 and 100mm lengths
Simultaneously separate the herbicides diquat and paraquat with Thermo ScientificTM AcclaimTM Trinity Q1 LC Columns. These tri-mode (WCX, WAX, RP) columns offer unmatched high-resolution and high-throughput trace analysis through LC-MS/MS and LC-UV methods. The stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, provides multiple retention mechanisms, including reversed-phase, anion-exchange, and cation-exchange, for maximum flexibility in method development. Easily optimize the selectivity to accelerate chromatographic separations and increase productivity. Superior Performance for Diquat and Paraquat Analysis • Distinctive spatial separation of anion-exchange and cation-exchange regions for unparalleled chromatographic performance • Adjustable selectivity using mobile phase buffer concentration, pH, and solvent content • Quick separation of hydrophilic ionic and ionizable analytes without ion-pairing reagent • Ideal selectivity for simultaneous separation of basic, neutral, and acidic analytes • Compatibility with mass spectrometry detection, which significantly improves sensitivity and provides conformation at the same time • Available in 3.0μm particle size and 2.1 and 3.0mm diameters, in 50mm length
Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns. Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations without the elevated backpressures required by sub-2μm particles. • Fast separations with excellent resolution and sensitivity • Lower backpressures so that UHPLC is not required • Mixed-Mode Selectivity for Aromatic and Moderately Polar Analytes • Enhanced Pi-pi Interactions with Aromatics • Moderate Hydrophobicity
Achieve fast, high-resolution separations of hydrophobic, long-chain compounds with Thermo Scientific™ Accucore™ C30 LC columns. Robust 2.6 µm solid-core particles enable rapid, high-resolution separations at low back pressures. The C30 stationary phase provides unique shape selectivity for structurally related isomers and is compatible with highly aqueous mobile phases. Solid binding technology and automated packing procedures ensure excellent reproducibility and long column life. Unique selectivity • High shape selectivity for hydrophobic, long chain and structurally related isomers Compatible with highly aqueous mobile phases • With a bonded phase, phase collapse does not occur in highly aqueous mobile phases. Quick breakups • 2.6 µm particles with Core Enhanced Technology enable operation at high flow rates without creating excessive back pressures. Excellent peak shapes for improved resolution and sensitivity • 2.6 µm solid core particles and narrow particle size distribution reduce band broadening and improve separation efficiency. • High surface coverage of silica minimizes secondary interactions and peak tailing. Low back pressures • 2.6 µm solid core particles for less back pressure compared to UHPLC particles under 2 µm • Compatible with traditional HPLC instrumentation and UHPLC platforms Excellent reproducibility and robustness • Advanced binding technology • Automated packing process
Hypersil GOLD 5µm 10x8mm Prep Column Guard
Achieve highly efficient separations of hydrophobic, ionizable compounds with Thermo Scientific™ Dionex™ IonPac™ NS1 10 µm analytical and guard columns. These polymer-based reversed-phase columns offer excellent resolution and peak shape and are the columns of choice for ion pair or ion suppression chromatography. The packing is a neutral, macroporous, high surface area ethylvinylbenzene polymer cross-linked with 55% divinylbenzene, making IonPac NS1 columns resistant to solvents, acids and bases while allowing the use of eluents with pH values from 0 to 14. Polymer-based columns for efficient reverse phase separations • Ideal for the separation of large molecules with local charges such as: B. surfactants are suitable • Can be used for traditional reverse phase polymeric applications • For ion pair chromatography for difficult separations • Available in particle sizes of 5 and 10 μm
The high-performance BioBasic™ 4 LC columns are designed for the analysis of larger proteins and peptides and are ideal for the analysis of proteins and peptides as well as other biomolecules with high hydrophobicity. • Based on 300Å silica for exceptional performance on biomolecules • Lower carbon load for optimal retention of larger peptides and proteins • Exceptionally high reproducibility, effectiveness and column life • Excellent for LC/MS separations
Classic stationary Hypersil phases are the recognized industry standard for HPLC (high-pressure liquid chromatography). Classic Hypersil phases have proven to be an effective analytical tool, they are well established and are listed as a reference in many HPLC methods. Manufacturing according to ISO 9001-2000 guidelines guarantees the highest quality. • For separation of certain aromatic compounds and moderately polar compounds • Retention properties are similar to Hypersil MOS • Alternative selectivity for C8
Use this proven, reliable L1. Thermo Scientific™ Hypersil™ ODS-2 C18 HPLC Columns are an alternative to older brands of columns commonly referenced in validated methods. The Hypersil stationary phases are the recognized industry standard for HPLC (high-pressure liquid chromatography). Classic Hypersil phases have proven to be an effective analytical tool, they are well established and are listed as a reference in many HPLC methods. Manufacturing according to ISO 9001-2000 guidelines guarantees the highest quality. • Based on type A silica • Very good reproducibility • Selectivity over a wide range of applications
Hypersil™ SAX LC Columns are highly stable silica-based quaternary amine anion exchange columns designed for aqueous mobile phases and low pH mobile phases. Classic stationary Hypersil phases are recognized worldwide as the industry standard for HPLC and are an effective analytical tool. Introduced in 1976, Hypersil phases are well established and are used in many HPLC processes worldwide. Thermo Fisher Scientific is the sole manufacturer of Hypersil silica and bonded phases. • High stability towards aqueous mobile phases and mobile phases with low pH value • Suitable for the analysis of smaller organic molecules including nucleotides and organic acids
The OmniPac PCX-100 analytical column was developed to optimize the separation of hydrophobic and halogenated anions. This polymer-based anion exchange column is solvent compatible and stable over the entire pH range of 1 to 14. Solvents can be used to influence selectivity, dissolve otherwise insoluble samples, and remove contaminants to extend column life. The OmniPac PAX-100 column is particularly suitable for the analysis of larger organic acids, including alkylbenzene sulfonates, aromatic acids, barbiturates, polyphosphates, inositol phosphates, thiocyanates, thiosulfates and iodates. • Composed of a solvent-compatible polymer-based microporous substrate agglomerated with a latex bead layer that ensures anion exchange functionality • Ion exchange capacity of approx. 40 μeq/column • 100% solvent compatible; should always be operated with at least 1% organic solvent in the mobile phase
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