If you need to separate anionic and neutral species in a single run, use the Thermo Scientific™ OmniPac™ PAX-500 Column. With anion exchange and reverse phase properties, this polymer-based mixed mode column is solvent compatible and stable over the entire pH range of 1 to 14. Solvents can be used to influence selectivity and to remove contaminants to extend column life. The OmniPac PAX-500 column is ideal for analyzing a wide range of compounds. The OmniPac PAX-500 column is ideal for the analysis of alcohols, alkanolamines, antihistamines, anti-inflammatory agents, aromatic acids, inorganic acids, peptides, purines and pyrimidines, sulfonamides, sulfonated anionic surfactants and water-soluble vitamins. • Macroporous hydrophobic core provides reverse phase retention and is coated with an anion exchange latex bead layer for anion exchange retention • Ion exchange capacity of approx. 40 μeq/column • 100% solvent compatible; should always be operated with at least 1% organic solvent in the mobile phase
Separate cationic and neutral analytes in a single run with Thermo Scientific™ OmniPac™ PCX-500 columns. With cation exchange and reversed phase properties, this polymer-based mixed mode column is solvent compatible and stable over the entire pH range of 1 to 14. Solvents can be used to influence selectivity and to remove contaminants to extend column life. This column is ideal for the analysis of a wide range of compounds including adrenergies, alcohols, anilines, antidepressants, anti-inflammatory agents, plating bath brighteners, cephalosporins, dyes and dyes, herbicides, nucleotides and nucleosides, peptides, sulfonamides and water-soluble vitamins. • Macroporous, hydrophobic core provides reverse phase retention and is coated with a cation exchange latex bead layer for cation exchange retention • Ion exchange capacity of approx. 120 μeq/column • 100% solvent compatible; should always be operated with at least 1% organic solvent in the mobile phase
Separate hydrophobic cations with the solvent-compatible Thermo Scientific™ OmniPac™ PCX-100 column. This polymer-based cation exchange column is solvent compatible and stable over the entire pH range of 1-14. Solvents can be used to influence selectivity, dissolve otherwise insoluble samples, and remove contaminants to extend column life. The OmniPac PCX-100 column is particularly well suited for the analysis of hydrophobic cationic analytes such as amines, anilines, antidepressants, drugs and metabolites (such as methotrexate and folic acid). • Composed of a solvent-compatible polymer-based microporous substrate agglomerated with a latex bead layer that ensures cation exchange functionality • Ion exchange capacity of approx. 120 μeq/column • 100% solvent compatible; should always be operated with at least 1% organic solvent in the mobile phase
Feature strong anion-exchange resin for high-resolution analysis and purification of synthetic and modified oligonucleotides with Thermo Scientific™ Dionex™ DNAPac™ PA100 Oligonucleotide Columns. 13μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads™ Capable of n, n-1 resolution for oligonucleotides Resolves oligonucleotides with secondary structures Compatible with solvent, high pH, and high temperatures Analyzes phosphorothioate-based clinical samples Provides easy scale-up
Enjoy strong anion exchange for high-resolution analysis and purification of synthetic oligonucleotides with Thermo Scientific™ DNAPac™ PA200 Oligonucleotide HPLC Columns. • Achieve n, n-1 resolution for oligonucleotides • Resolve oligonucleotides with secondary structures • Assay phosphorothioate purity • Selectivity control with eluent pH, salt, and solvent • Resolve RNA with aberrant (2' – 5') links from normal SSRNA
Use Thermo Scientific™ ProPac™ WCX-10 LC Columns to provide exceptionally high resolution for separations of proteins and their variants. Based on stationary phases composed of 10μm nonporous polymeric beads, ProPac columns resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer grafted to the particle core eliminates unwanted secondary interactions. The cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity. Multiple Formats for Greater Flexibility ProPac WCX-10 column is a weak cation-exchanger with a carboxylate functional group Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semi-prep work Superior Performance for Protein Analysis Ideal for characterization and quality control assessment of MAbs and other proteins Well-suited for characterization of related protein variants including deamidation and MAb lysine truncation variants Unequaled high-resolution separations Unmatched column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing
Separate proteins and their variants using Thermo Scientific™ ProPac™ SCX-10 LC columns, which offer exceptionally high resolution. ProPac columns are based on stationary phases made from non-porous 10 µm polymer beads and resolve isoforms that only in a single charged residue. A thin, hydrophilic layer grafted onto the particle core eliminates unwanted secondary interactions. The cation exchange surface provides pH-based selectivity control and rapid mass transfer for highly efficient separation and moderate capacity. • Non-porous core particle results in highly efficient peaks and high resolution separations of protein variants • The hydrophilic layer prevents unwanted secondary interactions • Grafted cation exchange surface provides pH-based selectivity control and rapid mass transfer for highly efficient separation and moderate capacity • Suitable for characterizing related protein variants
ProPac™ SAX-10 LC Columns for exceptionally high resolution and efficiency in separating protein variants. ProPac columns are based on a non-porous 10 µm core particle that provides pressure stability and can resolve isoforms that only differentiate in a single charged residue. A thin, hydrophilic layer prevents unwanted secondary interactions. The grafted anion exchange surface provides pH-based selectivity control and rapid mass transfer for highly efficient separation and moderate capacity. Maximum resolution • The ProPac SAX-10 LC column is a strong anion exchanger with quaternary amine as a functional group • Column size ranges from 2 mm ID for analytical applications to 22 mm ID for semiprep applications Outstanding performance in protein analysis • Ideal for characterizing and assessing the quality control of closely related protein variants • Unparalleled high resolution separations • Highly efficient peaks with excellent recovery • Higher capacity than other non-porous particle based columns • Excellent reproducibility across columns and batches eliminates the problem of column variability in stability and QA/QC testing
ProPac™ WAX-10 LC columns for exceptionally high resolution and efficient anion exchange separation of protein variants. Based on a non-porous 10 µm core particle that provides pressure stability, ProPac columns can resolve isoforms that differ in only a single charged residue A thin, hydrophilic layer prevents unwanted secondary interactions. The grafted anion exchange surface provides pH-based selectivity control and rapid mass transfer for highly efficient separation and moderate capacity. Maximum resolution • The ProPac WAX-10 LC column is a weak anion exchanger with tertiary amine as a functional group. • Column size ranges from 2 mm ID for analytical applications to 22 mm ID for semiprep applications Outstanding performance in protein analysis • Ideal for characterizing and assessing the quality control of closely related protein variants • Unparalleled high resolution separations • Highly efficient peaks with excellent recovery • Higher capacity than other non-porous particle based columns • Excellent reproducibility across columns and batches eliminates the problem of column variability in stability and QA/QC testing
Use Thermo Scientific™ MAbPac™ SCX-10 LC Columns for high-resolution, high efficiency analysis of monoclonal antibodies (mAbs) and associated variants. The unique nonporous pellicular resin provides the resolving power to separate mAb variants that differ by as little as one charged residue. A hydrophilic layer surrounds the polymeric beads and eliminates hydrophobic interactions with the resin, resulting in very efficient peaks. A proprietary grafted cation-exchange surface provides pH selectivity control for high-resolution separations. Superior Performance for mAb Variant Analysis • Exceptionally high resolution for mAb variants • Ideal for characterization and quality control assessment of mAbs • Well-suited for stability studies, mAb therapeutic method development and high-throughput screening • Unmatched column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing • Meets the regulatory requirements for biopharmaceutical characterization Multiple Sizes Available • Available in 3, 5 and 10μm particle sizes in standard and UHPLC formats • Smaller-particle-size columns provide the same resolution as the 10μm columns, but with significantly faster analysis time
Easily scale up your analytical method using Thermo Scientific™ Hypersil™ PREP BDS C18 reversed phase preparative HPLC Columns. The prep columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. These endcapped, silica-based columns offer C18 retention with base deactivation for reduced silanol activity and are an excellent choices for routine applications which require development from analytical to preparative LC scale. Excellent Choice for Routine Applications • C18 (USP L1) selectivity. • Base deactivated silica. • pH stable from 2 to 8. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
Maintain retention for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil™ PREP BDS Phenyl reversed phase preparative HPLC columns as you scale up your method. The stationary phase is base deactivated for reduced silanol activity and has moderate hydrophobicity, making it ideal for the separation of analyte mixtures with varying polarity and aromaticity. These preparative HPLC columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Unique Selectivity • Enhanced selectivity for aromatic and moderately polar analytes. • Base deactivated silica. • Selectivity complementary to that provided by alkyl chain phases. • Moderate hydrophobicity compared to C18. • Phenyl (USP L11) selectivity. • pH stable from 2 to 8. • Maximum temperature of 60°C. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. THE Thermo Scientific™ preparative guard columns require the use of a preparative guard holder.
Provide outstanding size separation of water soluble compounds with Thermo Scientific™ BioBasic™ SEC 60 Size Exclusion Chromatography preparative HPLC Columns. BioBasic SEC columns provide high efficiency separations for a wide range of molecular weights from 100 to 60,000 daltons, allowing for the analysis of proteins, peptides and other biomolecules. BioBasic™ PREP SEC 60 HPLC Column Benefits: • Covers separation of analytes over a wide molecular weight range • Long column life and high column efficiencies • Simple mechanism of interaction based on molecular size and shape • Excellent for sample cleanup • Straightforward method development, simple mobile phases • 60Å pore size
Provide outstanding size separation of water soluble compounds with Thermo Scientific™ BioBasic™ SEC 120 Size Exclusion Chromatography preparative HPLC Columns. BioBasic SEC columns provide high efficiency separations for a wide range of molecular weights from 700 to 120,000 daltons, allowing for the analysis of proteins, peptides and other biomolecules. BioBasic™ PREP SEC 120 HPLC Column Benefits: • Covers separation of analytes over a wide molecular weight range • Long column life and high column efficiencies • Simple mechanism of interaction based on molecular size and shape • Excellent for sample cleanup • Straightforward method development, simple mobile phases • 120Å pore size
Provide outstanding size separation of water soluble compounds with Thermo Scientific™ BioBasic™ SEC 300 Size Exclusion Chromatography preparative HPLC Columns. BioBasic SEC columns provide high efficiency separations for a wide range of molecular weights from 7,000 to 1,200,000 daltons, allowing for the analysis of proteins, peptides and other biomolecules. BioBasic™ PREP SEC 300 HPLC Column Benefits: • Covers separation of analytes over a wide molecular weight range • Long column life and high column efficiencies • Simple mechanism of interaction based on molecular size and shape • Excellent for sample cleanup • Straightforward method development, simple mobile phases • 300Å pore size
Provide outstanding size separation of water soluble compounds with Thermo Scientific™ BioBasic™ SEC 1000 Size Exclusion Chromatography preparative HPLC Columns. BioBasic SEC columns provide high efficiency separations for a wide range of molecular weights from 70,000 to 10,000,000 daltons, allowing for the analysis of proteins, peptides and other biomolecules. BioBasic™ PREP SEC 120 HPLC Column Benefits: • Covers separation of analytes over a wide molecular weight range • Long column life and high column efficiencies • Simple mechanism of interaction based on molecular size and shape • Excellent for sample cleanup • Straightforward method development, simple mobile phases • 120Å pore size
Build off of conventional HPLC methods for your scale up purification needs by using Thermo Scientific™ Accucore™ XL C18 preparative HPLC Columns. Rugged 4μm solid-core particles ensure high-efficiencies within low pressure limits requirements. The high bonded phase coverage delivers optimal retention of a comprehensive range of non-polar analytes throughout numerous applications. These columns come in a wide range of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Optimal Selectivity for Nonpolar Compounds • Hydrophobic interaction mechanism. • C18 (USP L1) selectivity. High-Efficiency Separations for Enhanced Resolution and Sensitivity • Core-enhanced technology 4μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies for conventional HPLC methods. • High surface coverage of silica minimizes secondary interactions and peak tailing. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. The preparative guard columns require the use of a preparative guard holder.
Improve conventional HPLC methods for your scale up purification needs by using Thermo Scientific™ Accucore™ XL C8 preparative HPLC columns. Rugged 4μm solid-core particles guarantee high-efficiencies within standard HPLC pressure limits. These high coverage C8 bonded phase delivers optimal retention of analytes with moderate hydrophobicity. These columns come in a wide range of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Lower Hydrophobic Retention • Recommended for moderately hydrophobic analytes or when a less hydrophobic phase provides optimum retention. • C8 (USP L7) selectivity. High-Efficiency Separations for Enhanced Resolution and Sensitivity • Core-enhanced technology 4μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies for conventional HPLC methods. • High surface coverage of silica minimizes secondary interactions and peak tailing. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. The preparative guard columns require the use of a preparative guard holder.
Attain repeatable and consistent LC separations for your scale up purification needs with Thermo Scientific™ Syncronis™ C18 reversed phase preparative HPLC columns. These preparative HPLC columns have extremely pure, high surface area silica with dense stationary phase coverage to minimize secondary interactions and ensure robust, high-resolution separations. Double endcapping provides additional surface coverage and inertness toward basic compounds. Columns come in a variety of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Highly Reproducible Separations • Rigorously tested stationary phase bonded to the highest quality silica ensures consistent, predictable LC performance. Enhanced Resolution and Sensitivity • Dense surface coverage of silica minimizes secondary interactions and peak tailing. • Double endcapped for extra surface coverage. • C18 (USP L1) selectivity. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use. • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. These preparative guard columns require the use of a preparative guard holder.
Maintain consistent, predictable separations for your scale up purification needs with Thermo Scientific™ Syncronis™ HILIC preparative HPLC columns for polar and hydrophilic analytes. Highly pure, high surface area silica and dense stationary phase coverage minimize secondary interactions and deliver reproducible high-resolution separations. The zwitterionic stationary phase results in a neutral, highly polar surface and provides optional selectivity to C18 columns without ion-pairing or derivatization. These columns come in a wide range of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. Alternative Selectivity • Zwitterionic bonded phase enhances retention of polar and hydrophilic analytes. • No need for ion-pair or derivatization. • Enhanced sensitivity with MS detection. • Highly organic mobile phase facilitates desolvation in ESI-MS. Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs. Standard Threaded and Standard Flange Hardware • Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use • Standard threaded hardware for column i.d. < 40mm. • Standard flange hardware for column i.d. ≥ 40mm. • Standard hardware can be modified on request to be compatible with SFC. XtendedLife Hardware • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly. • Compatible with SFC and is available in dimensions up to 50mm i.d. Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. These preparative guard columns require the use of a preparative guard holder.
Please fill out your contact data and your information request, we will contact you as soon as possible with all requested information.
Please fill out your contact data and your information request, we will contact you as soon as possible with all requested information.
