ZORBAX Eclipse Plus columns are the recommended starting point for method development and benefit from the ultimate performance from fully porous silica-based columns
ZORBAX Eclipse Plus columns are the recommended starting point for method development and benefit from the ultimate performance from fully porous silica-based columns
Eclipse XDB Columns • provide excellent separations over a wide pH range (pH 2 to pH 9), • provide reliable performance under high pressure and flow - a result of Agilent's proprietary ZORBAX silica gel particles, • enable flexible method development with many choices in four bonded phases and a wide range of particle sizes and dimensions.
Eclipse XDB Columns • provide excellent separations over a wide pH range (pH 2 to pH 9), • provide reliable performance under high pressure and flow - a result of Agilent's proprietary ZORBAX silica gel particles, • enable flexible method development with many choices in four bonded phases and a wide range of particle sizes and dimensions.
PL-SAX for Biomolecules is ideal for the anion-exchange HPLC separations of proteins and deprotected synthetic oligonucleotides under denaturing conditions. The covalently linked strong anion exchange functionality extends the operating pH range, and the anion exchange capacity is independent of pH. For synthetic oligonucleotides, separations using denaturing conditions of temperature, organic solvent, and high pH are all possible. PL-SAX for Biomolecules delivers improved chromatography for self-complementary or G-rich sequences that may associate to form aggregates or hairpin structures. The 5 μm material provides high efficiency separations of n and n-1 sequences.
PL-SCX for Prep to Process PL-SCX strong cation-exchange materials are extremely hydrophilic and are designed for purification of biomolecules. The materials are totally polymeric and are chemically and thermally stable over a full range of HPLC conditions. The strong cation-exchange functionalities, covalently linked to a chemically stable polymer, facilitate cation-exchange purifications over a wider pH range. This stability can be exploited for column sanitation and clean-up, and also enables the use of denaturing conditions and stabilizing/solubilizing agents for the purification of target compounds, as encountered in the purification of synthetic oligonucleotides with self-complementary sequences.
GPC/SEC Columns. PolarGel GPC columns are designed for use with polar organic solvents such as DMF, DMSO, NMP, and DMAc, as well as water/organic mixtures. These eluents are very useful in GPC/SEC to separate polar polymers, such as resins, starches, acrylics, biopolymers, polysaccharides, polyamides, and other polar polymers that are insoluble in water or organic solvents.
GPC/SEC Columns. PolarGel GPC columns are designed for use with polar organic solvents such as DMF, DMSO, NMP, and DMAc, as well as water/organic mixtures. These eluents are very useful in GPC/SEC to separate polar polymers, such as resins, starches, acrylics, biopolymers, polysaccharides, polyamides, and other polar polymers that are insoluble in water or organic solvents.
Polaris - Reversed Phase HPLC Columns. Agilent Polaris HPLC columns offer many polar phase chemistries designed separate mixtures of polar and nonpolar analytes reliably and with excellent peak shapes. The polar-embedded C8 and C18 bonded phases are compatible with 100% water, and offer alternative selectivities while still complying with USP L1 and USP L7. The combination of high carbon load, ultrapure silica, and silanol shielding provides superior peak shape and retention with highly polar samples.
Polaris - Reversed Phase HPLC Columns. Agilent Polaris HPLC columns offer many polar phase chemistries designed separate mixtures of polar and nonpolar analytes reliably and with excellent peak shapes. The polar-embedded C8 and C18 bonded phases are compatible with 100% water, and offer alternative selectivities while still complying with USP L1 and USP L7. The combination of high carbon load, ultrapure silica, and silanol shielding provides superior peak shape and retention with highly polar samples.
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