Analytical EC column, particle size 5 μm, pore size 100 Å, 25 % C, USP L1. Crosslinked hydrophobic phase; polymeric modiication; inert towards acidic and basic substances with high ainity for silica pH stability 1–9 Carbon content 25 %; distinct steric selectivity
Analytical EC column, particle size 3 μm, pore size 100 Å, Nonpolar hydrophobic high density phases; monomeric modiication pH stability 2–9 Carbon content 20 %
Analytical EC column, particle size 5 μm, pore size 100 Å, Nonpolar hydrophobic high density phases; monomeric modiication pH stability 2–9 Carbon content 20 %
Analytical EC column, particle size 5 μm, pore size 100 Å, not endcapped, 8.5 % C, Nonpolar phases for RP and ion-pairing chromatography Endcapped and non-endcapped modiications available; pH stability at 20 °C: 2–8 · Carbon content depending on pore size (see table) Recommended application · Separation of moderately to highly polar (water-soluble) compounds: steroids, nucleosides, cyclodextrins, pharmacological plant constituents
Analytical EC column, NUCLEOGEN® 60-7 DEAE particle size 7 μm, pore size 60 Å. Base material silica, particle size 7 μm; DEAE anion exchanger · For the separation of oligonucleotides up to chain lengths of 40 bases with recoveries > 95 % capacity 200 A260/mL (~ 300 A260 for a 125 x 4 mm ID column, 1875 A260 for a 125 x 10 mm ID column) · Preparative separations possible when using higher low rates and longer gradient times
Analytical EC Column. RESOLVOSIL BSA-7 protein phase for enantiomer separation · USP L75 · Base material NUCLEOSIL® silica, particle size 7 μm, pore size 300 Å chiral selector bovine serum albumin (BSA) · Separation based on selective interaction of proteins with low molecular compounds, i.e. principles of bioainity, including hydrophobic interactions (similar to a true reversed phase), interactions of polar groups and steric efects Recommended application · Amino acid derivatives, aromatic amino acids, aromatic sulfoxides, barbiturates, benzodiazepinones, benzoin and benzoin derivatives, β-blockers, coumarin derivatives, and for monitoring stereoselective microbial and enzymatic conversions
Analytical EC column. NUCLEOCEL DELTA enantiomer separation based on a cellulose derivative · USP L40 Technical data · Base material silica, chiral selector cellulose tris-(3,5-dimethylphenylcarbamate) High resolution type (S) with 5 μm particle size, allows use of shorter columns (150 mm) for faster separations, pressure stability up to ~150 bar (2000 psi), pH stability 1–9 NUCLEOCEL DELTA for normal phase applications: eluent in column n-heptane – 2-propanol (90:10, v/v) typical eluents are heptane – propanol mixtures NUCLEOCEL DELTA-RP for reversed phase applications: eluent in column acetonitrile – water (40:60, v/v) designed for use either in polar organic mode or with eluents containing high concentrations of chaotropic salts such as perchlorate Recommended application · Pharmaceutically active compounds, chiral pollutants (e.g., herbicides, PCB), chiral compounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds
Analytical EC column. NUCLEOCEL DELTA enantiomer separation based on a cellulose derivative · USP L40 Technical data · Base material silica, chiral selector cellulose tris-(3,5-dimethylphenylcarbamate) High resolution type (S) with 5 μm particle size, allows use of shorter columns (150 mm) for faster separations, pressure stability up to ~150 bar (2000 psi), pH stability 1–9 NUCLEOCEL DELTA for normal phase applications: eluent in column n-heptane – 2-propanol (90:10, v/v) typical eluents are heptane – propanol mixtures NUCLEOCEL DELTA-RP for reversed phase applications: eluent in column acetonitrile – water (40:60, v/v) designed for use either in polar organic mode or with eluents containing high concentrations of chaotropic salts such as perchlorate Recommended application · Pharmaceutically active compounds, chiral pollutants (e.g., herbicides, PCB), chiral compounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds
Analytical EC Column .NUCLEODEX α-PM eluent methanol – 50 mmol/L phosphate pH 3 (70:30)
Analytical EC Column. NUCLEODEX β-OH eluent methanol – 0.1 % TEAA pH 4 (55:45)
Analytical EC Column. NUCLEODEX β-PM eluent methanol – 0.1 % TEAA pH 4 (65:35)
Analytical EC Column. NUCLEODEX γ-PM eluent methanol – 0.1 % TEAA pH 4 (55:45)
Analytical EC column, special RP phase with protective polar group Technical data RP phase with pronounced hydrophilic properties Monomeric coating Endcapped Carbon content 11 %, particle size 5 μm, pore size 100 Å
This analytical EC column is packed with not endcapped C8 (MOS, monomeric octyl silyl) modification, based on the original NUCLEOSIL silica gel.
This analytical EC column is packed with not endcapped C8 (MOS, monomeric octyl silyl) modification, based on the original NUCLEOSIL silica gel.
This analytical EC column is packed with not endcapped C8 (MOS, monomeric octyl silyl) modification, based on the original NUCLEOSIL silica gel.
This analytical EC column is packed with not endcapped C8 (MOS, monomeric octyl silyl) modification, based on the original NUCLEOSIL silica gel.
Analytical EC column, particle size 7 μm, pore size 100 Å, not endcapped, 8.5 % C
Analytical EC column, NUCLEOSIL® SA, USP L9. Strongly acidic cation exchanger (SCX) with benzenesulfonic acid modiication Capacity ~ 1 meq/g; pH stability at 20 °C: 2–8; carbon content 6.5 %, particle size 5 μm, pore size 100 Å
Preparative VarioPrep HPLC column NUCLEODUR C18 ec *** with adjustable Endfitting *** column octadecyl phase, particle size 5 μm, 17.5 % C , USP L 1, Pore size 110 Å
Preparative VarioPrep HPLC column NUCLEODUR C18 ec *** with adjustable Endfitting *** column octadecyl phase, particle size 10 μm, 17.5 % C, USP L1, Pore size 110 A
Analytical EC column, special octadecyl phase for PAH analysis · USP L1 Base material NUCLEOSIL® silica, particle size 5 μm, pore size 100 Å; polymeric coating Detection of the separated PAH with UV (250–280 nm), diode array or luorescence detection at diferent wavelengths for excitation and emission (acenaphthylene cannot be analyzed with luorescence detection) Recommended application Eicient gradient separation of the 16 PAHs according to EPA
Analytical EC column, USP L14, Strongly basic anion exchanger (SAX) with quaternary ammonium modiication Capacity ~ 1 meq/g; pH stability at 20 °C: 2–8; carbon content 10 %, particle size 5 μm, pore size 100 Å
Analytical EC column, USP L14, Strongly basic anion exchanger (SAX) with quaternary ammonium modiication Capacity ~ 1 meq/g; pH stability at 20 °C: 2–8; carbon content 10 %, particle size 10 μm, pore size 100 Å
Analytical EC column, particle size 5 μm, pore size 100 Å, octyl phase, USP L7 Nonpolar high density phases; monomeric modiication; endcapped; carbon content 13 % ·Corresponding NUCLEODUR® phases see C8 Gravity page 158 Recommended application Separation of moderate to strong polar (water soluble) analytes like steroids, cyclodextrines, pharmalogical plant ingredients
Analytical EC column, cyano phases · USP L10 Polar to midpolar cyano (nitrile) modiied silica pH stability at 20 °C: 2–8; carbon content 5 % for 100 Å pores, ~ 3 % for 120 Å pores Recommended application: Reversed phase and normal phase chromatography Normal phase: with low-polarity solvents for many compounds, which can also be separated on unmodiied silica, however, due to the rapid equilibration much more suitable for gradient separations Reversed phase: with diferent selectivity than C18, C8 or phenyl modiied packings
Vario-Prep HPLC column NUCLEODUR 100-5 C8 ec *** with adjustable Endfitting *** octyl phase, particle size 5 μm, 10.5 % C, USP L7
Vario-Prep HPLC column NUCLEODUR C18 Gravity*** with adjustable Endfitting *** octadecyl phase, 18 % C, USP L1
VarioPrep HPLC column NUCLEODUR C18 Pyramid, 5 µm *** with adjustable Endfitting ***phase for highly aqueous eluents · USP L1 · Stable in 100 % aqueous mobile phase systems · Interesting polar selectivity features · Excellent base deactivation; suitable for LC/MS due to low bleeding characteristics Technical data · Special phase with polar endcapping; pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm (7 and 10 μm particles for preparative purposes on request); carbon content 14 %; pH stability 1–9 Recommended application · Analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agents, organic acids
Analytical EC column. NUCLEODUR® CN / CN-RP cyano-modiied high purity silica phase · USP L10 Key feature · High retention capacity especially for very polar and unsaturated compounds · Multi-mode column (RP and NP) widens scope of selectivity · Stable against hydrolysis at low pH (working range pH 1–8) Technical data · Cyanopropyl-modiied high purity silica; pore size 110 Å; particle sizes 3 μm and 5 μm; carbon content 7 %; special endcapping · High reproducibility from lot to lot; diferent retention characteristics in comparison to C8 and C18 Recommended application · Tricyclic antidepressants, steroids, organic acids
NUCLEOSIL® 100-5 C18 MPN · USP L1 Key feature · Octadecyl phase, particle size 5 μm; pore size 100 Å · Dynamic protein binding capacity per g packing: 6 mg BSA, 110 mg cytochrome C · pH working range 2–8, max. working pressure 250 bar Technical data · Silica-based reversed phase materials with monomerically bonded alkyl chains, brush type structure predominantly hydrophobic forces with a small portion of hydrophilic interactions · Maximum separation eiciency can be achieved when the injected protein mass does not exceed 1–2 % of the maximum protein loading capacity.
NUCLEOSIL® 300-5 C4 MPN · USP L26 Key feature · Butyl phase, particle size 5 μm, pore size 300 Å · Dynamic protein binding capacity per g packing: 14 mg BSA, 27 mg cytochrome C especially suited for the puriication of larger, hydrophobic peptides and very diferent proteins · pH working range 2–8, max. working pressure 250 bar Technical data · Silica-based reversed phase materials with monomerically bonded alkyl chains, brush type structure predominantly hydrophobic forces with a small portion of hydrophilic interactions · Maximum separation eiciency can be achieved when the injected protein mass does not exceed 1–2 % of the maximum protein loading capacity.
NUCLEOSIL® 100-5 C18 PPN · USP L1 Key feature · Octadecyl phase, particle size 5 μm, pore size 100 Å, dynamic protein binding capacity per g packing: 8 mg BSA, 64 mg cytochrome C; suited for the separation of peptides and proteins up to about 40 kD, also suited for basic peptides Technical data · Silica-based reversed phase materials with polymerically bonded alkyl chains; exclusively hydrophobic interactions · pH working range 1–9, max. working pressure 250 bar
NUCLEOSIL® 500-5 C18 PPN · USP L1 Key feature · Octadecyl phase, particle size 5 μm, pore size 500 Å, dynamic protein binding capacity per g packing: 22 mg BSA, 40 mg cytochrome C; especially suited for large peptides and medium-size hydrophilic proteins Technical data · Silica-based reversed phase materials with polymerically bonded alkyl chains; exclusively hydrophobic interactions · pH working range 1–9, max. working pressure 250 bar
Analytical EC column, cyano phases · USP L10 Polar to midpolar cyano (nitrile) modiied silica pH stability at 20 °C: 2–8; carbon content 5 % for 100 Å pores, ~ 3 % for 120 Å pores Recommended application Reversed phase and normal phase chromatography Normal phase: with low-polarity solvents for many compounds, which can also be separated on unmodiied silica, however, due to the rapid equilibration much more suitable for gradient separations Reversed phase: with diferent selectivity than C18, C8 or phenyl modiied packings. Particle size 5 μm, pore size 100 Å; eluent in column acetonitrile – water
NUCLEOSIL® phenyl phases (C6H5) · USP L11 Relatively nonpolar, non-endcapped phases for RP and ion pairing chromatography Polarity similar to C8, but with diferent selectivity for PAHs, polar aromatics, fatty acids etc. · pH stability at 20 °C: 2–8; carbon content 8 % Recommended application · Separation of moderately polar compounds
Analytical EC column NUCLEOSIL C4 particle size 5 μm, pore size 300 Å. Butyl phases (C4) · USP L26. Endcapped phases for RP and ion-pairing chromatography pH stability at 20 °C: 2–8; carbon content ~ 2 % Retention times are shorter than on C8 and C18 phases Recommended application For separation of macromolecules and hydrophobic substances
Analytical EC column NUCLEOSIL C4 particle size 5 μm, pore size 120 Å. Butyl phases (C4) · USP L26. Endcapped phases for RP and ion-pairing chromatography pH stability at 20 °C: 2–8; carbon content ~ 2 % Retention times are shorter than on C8 and C18 phases Recommended application For separation of macromolecules and hydrophobic substances
Analytical EC columns. NUCLEOSIL® Anion II eluent 0.15 mol/L (NH4)₂HPO4 bufer pH 5.2. Technical data: · Base material NUCLEOSIL® silica, particle size 10 μm, pore size 300 Å strongly basic anion exchanger, exchange capacity 50 μeq/g, pH stability 2–7.5 · Eluent in column 0.15 mol/L (NH4 )2HPO4 bufer pH 5.2 recommended bufer concentration for separation of inorganic anions: 2 mmol/L phthalate · Preferred method of detection: conductivity or negative UV detection
VarioPrep HPLC column NUCLEODUR C18 Htech, 10 µm *** with adjustable Endfitting *** base-deactivated preparative octadecyl phase · USP L1 Key feature · Reliable and durable standard RP phase for up-scaling to preparative scale, suited for LC/MS · High loading capacity and excellent stability · Outstanding base deactivation Technical data · High density octadecyl modiication (C18); pore size 110 Å; particle sizes 1.8 μm, 3 μm, 5 μm, 7 μm and 10 μm for analytical and preparative separations; carbon content 18 %, pH stability 1–11 Recommended application · Sophisticated analytical and preparative separations of basic, neutral and acidic pharmaceuticals, derivatized amino acids, pesticides, fat-soluble vitamins, aldehydes, ketones and phenolic compounds
Analytical EC column. Pore size 110 Å; amino phase, 2.5 % C
Analytical EC column. NUCLEODUR® CN / CN-RP cyano-modiied high purity silica phase · USP L10 Key feature · High retention capacity especially for very polar and unsaturated compounds · Multi-mode column (RP and NP) widens scope of selectivity · Stable against hydrolysis at low pH (working range pH 1–8) Technical data · Cyanopropyl-modiied high purity silica; pore size 110 Å; particle sizes 3 μm and 5 μm; carbon content 7 %; special endcapping · High reproducibility from lot to lot; diferent retention characteristics in comparison to C8 and C18 Recommended application · Tricyclic antidepressants, steroids, organic acids
Analytical EC column. Unmodified NUCLEODUR® Pore size 110 Å; unmodified; eluent in column n-heptane
Analytical EC column. Unmodified NUCLEODUR® Pore size 110 Å; unmodified; eluent in column n-heptane
Analytical EC column. NUCLEOSIL® Carbohydrate separation of mono- and disaccharides · USP L8 Technical data · Matrix: NUCLEOSIL® silica with amino modiication, particlesize 10 μm Recommended application · RP separation of mono- and disaccharides
Analytical EC column. NUCLEOSIL® CHIRAL-1 enantiomer separation based on ligand exchange · USP L32 Technical data · Base material NUCLEOSIL® silica, particle size 5 μm, pore size 120 Å chiral selector L-hydroxyproline – Cu2+ complexes · Principal interaction mode: · formation of ternary mixed-ligand complexes with Cu(II) ions; diferences in the stability of the diastereomeric complexes cause chromatographic separation Recommended application · Enantiomers with two polar functional groups with the correct spacing such as α-amino acids, α-hydroxycarboxylic acids (e.g., lactic acid), N-alkyl-α-amino acids etc.
Analytical EC column. NUCLEOSIL® CHIRAL-2 · enantiomer separation in organic eluent systems · USP L36
Analytical EC column. NUCLEOSIL®· CHIRAL-3 enantiomer separation in organic eluent systems · USP L36
Analytical EC column. Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions. These HPLC columns are packed with polar aminopropyl modification, based on the original NUCLEOSIL silica gel.
Analytical EC column. C18 HPLC phase for PAH analysis. Based on the original NUCLEOSIL silica gel.
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