Guard filter for HK series. Removes insoluble components in the sample
Guard filter for HK series. Removes insoluble components in the sample
The Shodex Asahipak ODP-50 series are columns for reversed phase chromatography. These columns are packed with spherical porous polymer having octadecyl (C18) groups, and perform similar to general silica-based C18 ODS columns. These columns are compatible with not only water or polar organic solvents, but also the wide pH range between 2 to 13. There are ideal for LC-MS/MS analysis. • Relatively large pore size of 250 A is suitable for the analysis of amino acids, peptides, and proteins • Usable in a wide pH range from pH 2 to 13 • Usable in 100 % water and buffer solution • Best used for the analysis of basic substances • Fulfills USP L67 requirements • Also available as Asahipak C4P-50 with C4 groups • Also suitable for evaporative light scattering detector (ELSD), corona charged aerosol detector (CAD), and mass spectrometry (LC-MS/MS) Over 60% of all HPLC applications are Reversed Phase (RP) separations with C18 functional groups. Spherical silica particles proved their use as base material in HPLC columns for many years. To minimize the negative effects of residual silanol groups on the surface, researchers tried end-capping or polymeric coating of the particle and developed hybrid materials. Here we introduce an alternative for these ODS (octadecyl-bonded silica) RP columns: A polymer particle (for example polyvinyl alcohol) with 5 μm particle size is modified with C18 alkyl chains as functional groups and used as stationary phase. This HPLC column has greater long-term pH stability from 2 to 13. It contains no silanol groups and provides higher resolution for basic substances, for example drugs with tertiary amines (no peak tailing due to unwanted interactions). Also the polarity of the polymer gel is slightly more hydrophilic which gives more retention of polar compounds at the beginning of the chromatogram. The polymer-based RP material can be used in 100% water or buffer and a variety of organic solvents (isocratic and gradient separation). It has a pore size of 250 Å which makes it suitable for the analysis of proteins and peptides. The very low bleeding is advantageous for the hyphenation to mass spectrometry (MS) or particle-sensitive light scattering detectors. A C18 column with a stationary phase made of polymer offers more than double the life time compared to silica, because it is chemically more resistant. Shodex is specialized in polymer-based packing material for Reversed Phase chromatography. The modern column Asahipak ODP-50 (octadecyl-bonded polymer) combines all the advantages of a polymeric RP-C18 column. It is used for small organic molecules, pharmaceuticals, pesticides, hormones, proteins, vitamins and basic drugs.
Column for Polymer-based Reversed Phase Chromatography (Asahipak)
The Shodex ODP2 HP series offers polymer–based columns for reversed phase chromatography. The efficiency of ODP2 HP columns is improved over most resin-based columns and is comparable with that of silica-based ODS columns. They don't contain C18 functional groups, the separation comes from the particle itself. ODP2 HP has a better retention of highly polar substances compared to most general purpose silica-based C18 columns. Silica-based C18 ODS columns are susceptible to protein adsorption, resulting in degradation of the column. ODP2 HP is designed to exclude protein and thus, ODP2 HP can be used for analysis of drugs in biological samples containing protein without rapid column deterioration. As ODP2 HP can be used with low salt concentration without loss of peak shapes, it is excellent for LC/MS analysis. • Provides a large theoretical plate number nearly twice as much as generally available polymer-based reversed phase columns do • Offers enhanced retention of high polar substances compared to ODS columns • Suitable for the analysis of small molecules such as pharmaceuticals in the presence of protein matrix • Ideal for LC/MS analysis of high polar compounds • Fulfills USP L39 requirements The material is completely silanol-free and therefore proteins are not adsorbed in the column.
Column for Ion Exchange Chromatograpgy •Suitable for the analysis of relatively high molecular weight compounds, such as proteins, peptides, DNA, and RNA. •Usable under wide pH range between 2 and 12.
Organic SEC (GPC) Columns (General Analysis): THF Features: • Standard organic solvent SEC (GPC) column • Supports a wide range of applications from low to high molecular weight compounds • Fulfills USP L21 requirements
Organic SEC (GPC) Column (General Analysis): Dimethylformamid (DMF) Features: • Standard organic solvent SEC (GPC) column • Supports a wide range of applications from low to high molecular weight compounds • Fulfills USP L21 requirements
Polymer-based Hydrophilic Interaction Chromatography (HILIC) Column (HILICpak) • Suitable for saccharide analysis using HILIC mode • Recovers reducing saccharides with high ratio • Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period • Easily regenerated by washing in an alkaline solution • Appropriate for evaporative light scattering detector, corona charged aerosol detector, and LC/MS.
Polymer-based Hydrophilic Interaction Chromatography (HILIC) Column (HILICpak) • Suitable for anionic substances (especially phosphate compounds) analysis using HILIC mode • Use of some eluents add ion exchange mode • Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period • Suitable for LC/MS analysis
Polymer-based Hydrophilic Interaction Chromatography (HILIC) Column (HILICpak) • Modified carboxyl group is suitable for cationic substance analysis including amines • The dominant separation mode is RP or IEX rather than HILIC mode
Polymer-based Hydrophilic Interaction Chromatography (HILIC) Column (HILICpak) • The modified diol groups on the packing material create the HILIC mode • Suitable for oligosaccharide and oligonucleotide separation which is not possible by SEC column or conventional HILIC columns • PEEK housing (inner surface) to avoid interaction with the analytes and steel (outer surface) for stability
Column for Ligand Exchange Chromatography. Ligand exchange and HILIC
Columnfor Ion Exclusion Chromatography. For organic acids, cyanide ions and cyanogen chloride.
Chiral Separation Column. • Separates optical isomers by using their conformational compatibility differences • Versatile column for chiral separation • Fulfills USP L45 requirements.
Aqueous SEC (GFC) Column: Polymer-based • Polymer-based packed columns for aqueous SEC (GFC) analysis • Supports a wide range of molecular weight sample analysis • The eluent can be replaced with DMF (except SB-802 HQ and SB-807 HQ), enabling the analysis of polar polymers • Method using SB-804 HQ or SB-805 HQ for gelatin’s mean molecular weight determination is comparable with PAGI method (Ver. 10, Japan) • Fulfills USP L38 and L39 requirements • SB-802 HQ fulfills USP L25 requirements • SB-802.5 HQ fulfills USP L25 and L89 requirements • SB-803 HQ fulfills USP L37 requirements • SB-807 HQ Column for the analysis of water-soluble ultra high molecular weight polymers. Large particle-size gel prevents shear degradation of polymers. Fulfills USP L38 and L39 requirements
Column for Ion Exchange Chromatograpgy •Suitable for the analysis of relatively high molecular weight compounds, such as proteins, peptides, DNA, and RNA. •Usable under wide pH range between 2 and 12.
Column for Ion Exchange Chromatograpgy •Suitable for the analysis of relatively high molecular weight compounds, such as proteins, peptides, DNA, and RNA. •Usable under wide pH range between 2 and 12.
Column for Ion Exchange Chromatograpgy •Suitable for the analysis of relatively high molecular weight compounds, such as proteins, peptides, DNA, and RNA. •Usable under wide pH range between 2 and 12.
Column for weak anion exchange resin. Functional Group : Diethylaminoethyl.
Column for weak anion exchange resin. Functional Group : Carboxymethyl.
Column for Ion Chromatography (Anion Analysis). • Suitable for anion suppressor methods with sodium carbonate eluent • Suitable for the quantitative analysis of fluoride ion • SI-50 4E separates target inorganic anions from organic acids • SI-52 4E provides simultaneous analysis of oxyhalides and general inorganic ions • Carbonate peak does not interfere with analysis
IC NI-424 columns with quaternary ammonium The IC columns for anion analysis (non-suppressor method) • Ideal for anion non-suppressor methods • IC NI-424 provides simultaneous analysis of fluoride and phosphate ions
IC I-524A columns with quaternary ammonium The IC columns for anion analysis (non-suppressor method) • Ideal for anion non-suppressor methods • IC I-524A fulfills USP L23 requirements
• Column for cation analysis with non-suppressor method • Simultaneous analysis of monovalent and divalent cations • Suitable separating of alkylamines • Fulfills USP L76 requirements
Column for cation analysis • High performance type of YK-421 • Applicable to both suppressor and non-suppressor methods • Provides sharp peaks; more significant for divalent cation analysis • Supports the analysis of alkylamines and transition metals
Polymer-based RP columns for many different applications. The RSpak DE, DM and DS columns don't contain C18 as functional group, but they provide the reversed phase separation mechanism by the polymer particle itself. This makes them very stable, so the columns have a very long life time. RSpak DS-413 and DS-613 • Suitable for reversed phase analysis of highly hydrophilic substances that are not well retained by ODS columns • Fulfill USP L21 requirements (styrene divinylbenzene copolymer particles)
Polymer-based RP columns for many different applications. The RSpak DE, DM and DS columns don't contain C18 as functional group, but they provide the reversed phase separation mechanism by the polymer particle itself. This makes them very stable, so the columns have a very long life time. RSpak DE-213, DE-413 and DE-613 • General purpose polymer-based column having similar polarity as ODS columns • Wide working pH range (from pH 2 to 12), usable in 100 % water and buffer solutions • extra small pore size of 25 Å • for the analyis of vitamins, preservatives, organic acids • Fulfill USP L71 requirements (polymethacrylate particles)
Polymer-based RP columns for many different applications. The RSpak DE, DM and DS columns don't contain C18 as functional group, but they provide the reversed phase separation mechanism by the polymer particle itself. This makes them very stable, so the columns have a very long life time. RSpak DM-614 • Suitable for the analysis of amino acids and water-soluble vitamins • pH range from 2 to 10 • Fulfills USP L39 requirements (polyhydroxymethacrylate particles)
Column for Polymer-based Reversed Phase Chromatography (RSpak)
Column for Polymer-based Reversed Phase Chromatography (RSpak)
Column for Polymer-based Reversed Phase Chromatography (RSpak)
Aqueous SEC (GFC) Column: Polymer-based • Polymer-based packed column for aqueous SEC (GFC) analysis • Low column bleeding allows its use with light scattering detectors • The eluent can be replaced with DMF enabling the analysis of polar polymers • LB-804 (exclusion limit: about 1,000,000) and LB-806 (exclusion limit: about 20,000,000) newly added to the series • Fulfills USP L38 and L39 requirements • LB-803 fulfills USP L37 requirements
Aqueous SEC (GFC) Column: Silica-based • Silica-based packed column for aqueous SEC (GFC) analysis • Suitable for the analysis of proteins and enzymes • Fulfills USP L20, L33, and L59 requirements
Aqueous SEC (GFC) Column: Silica-based • Reduced packing material particle size enhances column performance • Three to four-fold higher sensitivity than KW-800 series • KW405-4F is applicable analyzing samples with molecular weight above 1,000,000 • Fulfills USP L20, L33, and L59 requirements
Aqueous SEC (GFC) Column: Silica-based. • Pore size specifically controlled for analyzing proteins with a molecular weight of several hundred of thousand • High performance analysis of antibody drugs and various proteins • High lot-to-lot reproducibility • Fulfills USP L20, L33, and L59 requirements
Aqueous SEC (GFC) Column: Silica-based. • Rapid analysis column for LW-803 • Achieves approximately halved analysis time compared with standard column • Fulfills USP L20, L33, and L59 requirements
Multimode Column. • SEC is the main separation mode • With the choice of eluent, the column provides multimode features of reversed phase, HILIC, and ion exchange modes to SEC • Suitable for the separation of peptides or nucleic acids with similar molecular weights • Suitable for desalting samples or substituting buffer in protein analysis
Aqueous/Organic SEC Column. Features: • Polymer-based SEC columns with high solvent durability • Works well with both aqueous and organic solvents
Ion Exclusion Chromatography Columns • Columns for simultaneous analysis of saccharides and organic acids • Separates neutral sugars in size exclusion mode and organic acids in ion exclusion mode • Suitable for the analysis of uronic and aldonic acids • Fulfill USP L17 and L22 requirements
Column for Ligand Exchange Chromatography.
Column for Ligand Exchange Chromatography. Ligand exchange and HILIC
SUGAR KS-800 columns with Sulfo (Na+) groups The Ligand Exchange column for mono-, oligo- and poly-saccharides SUGAR KS-801 and KS-802 • Separates mono- and oligo-saccharides by combination of ligand exchange (Na+) and size exclusion mode • Only water is required for the analysis of neutral sugars KS-803 to 804 • Suitable for separation of polysaccharides by size exclusion mode • Can be used in combination with other columns e.g., KS-802 and KS-801 • Only water is required for the analysis of neutral sugars • Fulfill USP L22 and L58 requirements These columns are mainly used in routine quality controls in food analysis. This column series has similar properties like the following references: BioRad Aminex HPX-87N (Na+), Agilent Hi-Plex PL Na, Phenomenex Rezex RNM-Carbohydrate Na+, Sepax Carbomix Na, Thermo Fisher HyperREZ XP Carbyhydrate Na+, Transgenomic Carbo Sep Coregel
Polymer-based Hydrophilic Interaction Chromatography (HILIC) Column (Asahipak)
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