Guard filter for HK series. Removes insoluble components in the sample
Guard filter for HK series. Removes insoluble components in the sample
The Shodex Asahipak ODP-50 series are columns for reversed phase chromatography. These columns are packed with spherical porous polymer having octadecyl (C18) groups, and perform similar to general silica-based C18 ODS columns. These columns are compatible with not only water or polar organic solvents, but also the wide pH range between 2 to 13. There are ideal for LC-MS/MS analysis. • Relatively large pore size of 250 A is suitable for the analysis of amino acids, peptides, and proteins • Usable in a wide pH range from pH 2 to 13 • Usable in 100 % water and buffer solution • Best used for the analysis of basic substances • Fulfills USP L67 requirements • Also available as Asahipak C4P-50 with C4 groups • Also suitable for evaporative light scattering detector (ELSD), corona charged aerosol detector (CAD), and mass spectrometry (LC-MS/MS) Over 60% of all HPLC applications are Reversed Phase (RP) separations with C18 functional groups. Spherical silica particles proved their use as base material in HPLC columns for many years. To minimize the negative effects of residual silanol groups on the surface, researchers tried end-capping or polymeric coating of the particle and developed hybrid materials. Here we introduce an alternative for these ODS (octadecyl-bonded silica) RP columns: A polymer particle (for example polyvinyl alcohol) with 5 μm particle size is modified with C18 alkyl chains as functional groups and used as stationary phase. This HPLC column has greater long-term pH stability from 2 to 13. It contains no silanol groups and provides higher resolution for basic substances, for example drugs with tertiary amines (no peak tailing due to unwanted interactions). Also the polarity of the polymer gel is slightly more hydrophilic which gives more retention of polar compounds at the beginning of the chromatogram. The polymer-based RP material can be used in 100% water or buffer and a variety of organic solvents (isocratic and gradient separation). It has a pore size of 250 Å which makes it suitable for the analysis of proteins and peptides. The very low bleeding is advantageous for the hyphenation to mass spectrometry (MS) or particle-sensitive light scattering detectors. A C18 column with a stationary phase made of polymer offers more than double the life time compared to silica, because it is chemically more resistant. Shodex is specialized in polymer-based packing material for Reversed Phase chromatography. The modern column Asahipak ODP-50 (octadecyl-bonded polymer) combines all the advantages of a polymeric RP-C18 column. It is used for small organic molecules, pharmaceuticals, pesticides, hormones, proteins, vitamins and basic drugs.
Column for Polymer-based Reversed Phase Chromatography (Asahipak)
The Shodex ODP2 HP series offers polymer–based columns for reversed phase chromatography. The efficiency of ODP2 HP columns is improved over most resin-based columns and is comparable with that of silica-based ODS columns. They don't contain C18 functional groups, the separation comes from the particle itself. ODP2 HP has a better retention of highly polar substances compared to most general purpose silica-based C18 columns. Silica-based C18 ODS columns are susceptible to protein adsorption, resulting in degradation of the column. ODP2 HP is designed to exclude protein and thus, ODP2 HP can be used for analysis of drugs in biological samples containing protein without rapid column deterioration. As ODP2 HP can be used with low salt concentration without loss of peak shapes, it is excellent for LC/MS analysis. • Provides a large theoretical plate number nearly twice as much as generally available polymer-based reversed phase columns do • Offers enhanced retention of high polar substances compared to ODS columns • Suitable for the analysis of small molecules such as pharmaceuticals in the presence of protein matrix • Ideal for LC/MS analysis of high polar compounds • Fulfills USP L39 requirements The material is completely silanol-free and therefore proteins are not adsorbed in the column.
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