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• Pre-assembled caps and septa reduce handling and therefore contamination.
• Aluminum crimp caps provide a leak-resistant seal.
• Use of a crimping tool is required for aluminum seals.
• Magnetic crimp seals for use with autosamplers.
• Snap caps are easy to apply and remove without tools, eliminating the need for crimping tools.
• Pre-assembled caps and septa reduce handling and therefore contamination.
• Aluminum crimp caps provide a leak-resistant seal.
• Use of a crimping tool is required for aluminum seals.
• Magnetic crimp seals for use with autosamplers.
• Snap caps are easy to apply and remove without tools, eliminating the need for crimping tools.
• Pre-assembled caps and septa reduce handling and therefore contamination.
• Aluminum crimp caps provide a leak-resistant seal.
• Use of a crimping tool is required for aluminum seals.
• Magnetic crimp seals for use with autosamplers.
• Snap caps are easy to apply and remove without tools, eliminating the need for crimping tools.
• Pre-assembled caps and septa reduce handling and therefore contamination.
• Aluminum crimp caps provide a leak-resistant seal.
• Use of a crimping tool is required for aluminum seals.
• Magnetic crimp seals for use with autosamplers.
• Snap caps are easy to apply and remove without tools, eliminating the need for crimping tools.
• Pre-assembled caps and septa reduce handling and therefore contamination.
• Aluminum crimp caps provide a leak-resistant seal.
• Use of a crimping tool is required for aluminum seals.
• Magnetic crimp seals for use with autosamplers.
• Snap caps are easy to apply and remove without tools, eliminating the need for crimping tools.
Features and Benefits:
• Pore sizes to suit your analysis (1000 Å or 120 Å).
• Intermediate polarity C8 phase.
• Continuity of using HPLC for all separation needs simplifies your workflow.
• Facilitates the use of MS.
• Eliminate SDS-PAGE from your workflow.
Recommended Applications:
120 Å recommended for compounds too strongly retained on C18 phases, 1000 Å recommended for proteins.
Product Specifications:
0.15, 0.3, 1, 2.1, 3, and 4.6 mm ID columns available in 50, 100, 150, 250 mm lengths. PEEK coated, and PEEKsil®, column hardware available to reduce non-specific interactions when analyzing compounds with metal chelating activity.
Features and Benefits:
• Pore sizes to suit your analysis (1000 Å or 120 Å).
• Intermediate polarity C8 phase.
• Continuity of using HPLC for all separation needs simplifies your workflow.
• Facilitates the use of MS.
• Eliminate SDS-PAGE from your workflow.
Recommended Applications:
120 Å recommended for compounds too strongly retained on C18 phases, 1000 Å recommended for proteins.
Product Specifications:
0.15, 0.3, 1, 2.1, 3, and 4.6 mm ID columns available in 50, 100, 150, 250 mm lengths. PEEK coated, and PEEKsil®, column hardware available to reduce non-specific interactions when analyzing compounds with metal chelating activity.
GRAM columns can be used for organic GPC and SEC polymer analysis in polar organic solvents. These polyester copolymer-based GPC columns are available in a range of porosities, from 30 to 10,000 Å, and one linear/mixed-bed version to meet the needs for a variety of polymers with molar masses ranging from 100 to 50,000,000 Da, including for the analysis of polyurethane, polyimide, starches, cellulose, and other polar polymers.
GPC/SEC Standards.
Polyisobutylene standards are well suited for organic GPC/SEC system and column calibration. Standards are offered as individual molecular weight standards from 112 to 700,000 Da. For convenience, a polyisobutylene calibration kit is also available with a range of molar masses from 400 to 700,000 Da
GPC/SEC Standards.
Polyacrylic acid (PAA) individual molecular weight polymer standards offer well-characterized Mp values to ensure wide utility for aqueous GPC/SEC column calibration. PAA standards are compatible with all aqueous columns for wide applicability. These aqueous polymer standards are available with molecular weights from 1,000 to 2,000,000 Da.
POLEFIN columns are useful for high-temperature organic GPC/SEC analysis of polyolefins with molecular weights from 100 to 30,000,000 Da. These organic GPC columns are based on a modified styrene-divinylbenzene copolymer network and have a maximum temperature rating of 210 °C. POLEFIN GPC columns offer flexible configurations, including two particle sizes and two different linear/mixed-bed columns to meet the needs for a variety of polymer analyses, such as polyethylene, polypropylene, and other polyolefins.
Agilent WinGPC 2D-GPC/SEC Analysis Add-On. Adds 2D-GPC/SEC calculation functionality to WinGPC Software. Not for standalone installation. Requires a WinGPC Software main Edition and an additional timebase (G7896AA). Not compatible with Client/Sever setup (G7890AA/AB only)
ReadyCal Kit PMMA low, nominal Mp 202 - 71,800 Da. Includes 10 each of 3 different vials containing preweighed standards with 4 different molar masses, 3 x 10 vials, 1.5 mL
Calibration kit Polystyrene low, nominal Mp 162 - 62,300 Da. Includes 8 different Polystyrene individual molar mass standards, nominal Mp 160 - 60,000 g/mol, 8 x 1.0 g
Polystyrene standards are the first choice for many organic solvents, either for conventional GPC/SEC column calibration or for calibrating light scattering and viscometry detectors. The molecular weights of our organic polymers are selected to provide equidistant calibration points for greater accuracy. Individual molecular weight standards are available from 162 to 15,000,000 Da, with options for broad molar mass distribution standards. Convenient calibration kits are also available, including traditional kits, InfinityLab EasiCal kits, and ReadyCal kits.
Calibration kit Poly(L-lactic acid), nominal Mp 358 - 75,600 Da. Includes 7 different Poly(L-lactic acid) individual molar mass standards, nominal Mp 500 - 72,000 g/mol, 7 x 0.2 g
Eluent filters, 4µm pore size. 10 PTFE eluent filters for mobile phase reservoir, with adapter for connection to tubing. Suitable for common GPC/SEC solvents including THF, Chloroform, DMF, DMAc, and aqueous solutions.
ReadyCal Kit Pullulan high, nominal Mp 180 - 1,450,000 Da. Includes 5 each of 3 different vials containing preweighed standards with 3 different molar masses, 3 x 5 vials, 1.5 mL
Calibration kit Polyacrylic acid sodium salt, nominal Mp 1,200 - 1,390,000 Da. Includes 10 different polyacrylic acid sodium salt individual molar mass standards, nominal Mp 1,200 - 1,400,000 g/mol, 10 x 0.25 g
Calibration kit Dextran, nominal Mp 180 - 225,000 Da. Includes 10 different Dextran individual molar mass standards, nominal Mp 180 - 225,000 g/mol, 10 x 0.25 g
Calibration kit Pullulan, nominal Mp 342 - 739,000 Da. Includes 10 different Pullulan individual molar mass standards, nominal Mp 340 - 700,000 g/mol, 10 x 0.1 g
InfinityLab Poroshell 120 HILIC columns for hydrophilic interaction liquid chromatography (HILIC) are designed for the retention and high-resolution separation of small polar analytes. The superficially porous character of the particles decreases equilibration times to improve throughput for both isocratic and gradient separations. Agilent offers three unique, orthogonal HILIC phases: bare silica (HILIC), polyhydroxy fructan (HILIC-OH5), and zwitterionic (HILIC-Z).
The robustness and reproducibility of HILIC separations on Poroshell columns help to switch from challenging, often MS-incompatible ion pair chromatography and normal phase applications.
Calibration kit Protein, nominal Mp 243 - 670,000 Da. Includes 10 different Protein individual molar mass standards, nominal Mp 240 - 670,000 g/mol, 10 x 0.1 g
PFG columns are well suited for the organic GPC/SEC analysis of crystalline polymers with molecular weights from 100 to 3,000,000 Da using fluorinated organic solvents. These polar modified silica-based columns are available in two particle sizes, 5 and 7 μm, and four single porosities, 100, 300, 1,000, and 4,000 Å, as well as three different linear/mixed-bed columns, to meet the needs for a variety of organic GPC applications, like crystalline polymers, polyesters, polyamides, polylactides, and POM.
Columns produced under DIN EN ISO 9001 quality standards
Fittings and integrated diffusers provide low dead volume and efficient distribution of the sample across the entire chromatographic bed for optimal sample injection
ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series.
The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions.
The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation.
Features
• High efficiency and reproducibility with short analysis time
• Semi-prep and prep column dimensions
• Compatible with organic modifiers and denaturants
• Wide usable pH range (3-8)
ReadyCal Kit PMMA, nominal Mp 800 - 2,200,000 Da. Includes 10 each of 3 different vials containing preweighed standards with 4 different molar masses, 3 x 10 vials, 4 mL
ReadyCal Kit Polystyrene, nominal Mp 474 - 2,520,000 Da. Includes 10 each of 3 different vials containing preweighed standards with 4 different molar masses, 3 x 10 vials, 1.5 mL
Polystyrene standards are the first choice for many organic solvents, either for conventional GPC/SEC column calibration or for calibrating light scattering and viscometry detectors. The molecular weights of our organic polymers are selected to provide equidistant calibration points for greater accuracy. Individual molecular weight standards are available from 162 to 15,000,000 Da, with options for broad molar mass distribution standards. Convenient calibration kits are also available, including traditional kits, InfinityLab EasiCal kits, and ReadyCal kits.
PolarSil columns are based on polar modified silica and ideal for the organic GPC/SEC separation of low to medium molar mass resins and lignins with molecular weights from 100 to 1,000,000 Da using polar organic solvents. Multiple column configurations are available to match your specific application, including two particle sizes, 3 and 5 μm, and three porosities, 100, 300, and 1,000 Å as well as two different linear/mixed-bed columns.
Agilent WinGPC Light Scattering/Viscosity Add-On. Adds Light Scattering and Visometry calculation functionality to WinGPC Software. Not for standalone installation. Requires a WinGPC Software main Edition
ReadyCal Kit Protein, nominal Mp 243 - 670,000 Da. Includes 5 each of 3 different vials containing preweighed standards with 3 or 4 different molar masses, 3 x 5 vials, 1.5 mL
Polybutadiene standards are suitable for organic GPC/SEC column calibration. These individual polymer standards are available with narrow molar mass distribution ranges, with molecular weights from 110 to 1,000,000 Da. Standards are also available in a calibration kit.
Calibration kit Poly(dimethylsiloxane), nominal Mp 311 - 305,000 Da. Includes 8 different Poly(dimethylsiloxane) individual molar mass standards, 8 x 0.5 g
SDV columns are useful for a wide variety of polymer analyses in nonpolar and medium polar organic solvents. These GPC columns are made from a styrene-divinylbenzene copolymer network and are available in three particle sizes (3, 5, and 10 μm) and a range of porosities to meet many organic GPC and SEC polymer analysis needs, including for polystyrene, polyvinyl chloride, polycarbonate, elastomers, resins, and more. The molecular weight range for SDV columns is 100 to 30,000,000 Da. SDV columns meet USP L21.
Calibration kit Polyisoprene-1.4, nominal Mp 1,030 - 1,180,000 Da. Includes 10 different Polyisoprene individual molar mass standards, nominal Mp 1,000 - 1,100,000 g/mol, 10 x 1.0 g
Agilent WinGPC Copolymer Analysis Add-On. Adds Copolymer Analysis calculation capabilities to WinGPC Software (for composition analysis and calculations). Not for standalone installation. Requires a WinGPC Software main Edition
